重庆市3960例流感病毒阴性流感样病例肠道病毒检测及分型分析  

Detection and genotyping of enteroviruses in 3960 influenza-negative influenza-like illness cases in Chongqing

作　　者：彭靖尧[1] 罗肖怡楠 赵华[1] 谢武娟 凌华[1] 黄为[1] PENG Jingyao;LUO Xiaoyinan;ZHAO Hua;XIE Wujuan;LING Hua;HUANG Wei(Chongqing Municipal Key Laboratory for High Pathogenic Microbes,Chongqing Center for Disease Control and Prevention,Chongqing 400042,China)

机构地区：[1]重庆市疾病预防控制中心,高致病性病原微生物重庆市重点实验室,重庆400042

出　　处：《公共卫生与预防医学》2024年第1期53-56,共4页Journal of Public Health and Preventive Medicine

基　　金：重庆市科卫联合医学科研项目(2020FYYX095)。

摘　　要：目的了解肠道病毒(Enterovirus,EV)在重庆市流感病毒阴性流感样病例(Influenza-like illness,ILI)中的流行特征以及基因型分布,为重庆市EV防控提供科学依据。方法收集重庆市流感监测点送检流感病毒阴性ILI病例咽拭子标本,使用荧光RT-PCR检测EV,EV阳性样本扩增VP4区片段并测序分型。结果2021年1~12月共收到3960例流感病毒阴性ILI标本,检出EV阳性316例(7.98%);重庆市流感病毒阴性ILI病例全年均能检出EV,检出率较高月份为1月(11.60%)、4月(10.56%)、5月(11.79%)、6月(12.62%)、7月(10.33%),不同区域、不同性别、不同年龄组的ILI病例EV检出率差异有统计学意义(X^(2)=29.647,X^(2)=4.192,X^(2)=69.176,P<0.05);213例阳性EV被成功分型,包含EV-A、EV-B、EV-C的17个型别和鼻病毒B组(HRV-B)的5个型别,优势型别为CV-A4(32.86%)、CV-A2(23.00%)、CA-6(12.21%)、CA-10(11.74%),未鉴定出EV-D和新型EV。结论EV是重庆市ILI病例的重要病原体,重庆市ILI病例中流行的EV具有典型区域性、季节性、以及人群特点,应根据EV流行特征在重庆地区开展防控工作。Objective To understand the epidemiological characteristics and genotype distribution of enterovirus(EV)in influenza-negative influenza-like illness(ILI)cases in Chongqing,and to provide a scientific basis for EV prevention and control.Methods Throat swab samples of influenza-negative ILI cases were collected from surveillance sites.The samples were detected for EV using real-time RT-PCR.The VP4 regions of positive samples were amplified and sequenced for genotyping.Results A total of 3960 influenza-negative ILI samples were collected from January to December 2021,and 316(7.98%)of them were EV-positive.EV could be detected in influenza-negative ILI cases in Chongqing all year round.The months with high EV-positive rates were January(11.60%),April(10.56%),May(11.79%),June(12.62%),and July(10.33%).There was a statistically significant difference in the detection rate of EV in ILI cases in different regions,gender,and age groups(X^(2)=29.647,X^(2)=4.192,X^(2)=69.176,P<0.05).A total of 213 EV-positive cases were successfully genotyped,including 17 genotypes of EV-A,EV-B,and EV-C and 5 genotypes of HRV-B.The dominant genotypes were CV-A4(32.86%),CV-A2(23.00%),CA-6(12.21%),and CA-10(11.74%).EV-D and novel EV were not identified in this study.Conclusion EV is an important pathogen in ILI cases in Chongqing.The prevalence of EV in ILI cases in Chongqing has typical regional,seasonal and population characteristics.Prevention and control should be carried out in Chongqing according to the epidemic characteristics of EV.

关 键 词：肠道病毒 流感样病例 VP4区 基因分型 

分 类 号：R181[医药卫生—流行病学]