金刚烷胺在大鼠体内的死后再分布研究  

Postmortem redistribution of amantadine in rats

作　　者：王锦凯 李文艳 刘唯琛 王振华[4] 任飞[5] 张潮[1,2,3] Wang Jinkai;Li Wenyan;Liu Weichen;Wang Zhenhua;Ren Fei;Zhang Chao(School of Forensic Medicine,Shanxi Medical University Jinzhong,Shanxi,030600;Shanxi Key Laboratory of Forensic Medicine,Jinzhong,Shanxi,030600;Key Laboratory of Forensic Toxicology of Ministry of Public Security,Jinzhong,Shanxi,030600;Xi'an Public Security Bureau,Xi'an,Shanxi,710000;Taiyuan Public Security Bureau,Taiyuan,Shanxi,030000)

机构地区：[1]山西医科大学法医学院,山西晋中030600 [2]山西省法医学重点实验室,山西晋中030600 [3]法庭毒物分析公安部重点实验室,山西晋中030600 [4]西安市公安局,陕西西安710000 [5]太原市公安局,山西太原030000

出　　处：《中国法医学杂志》2023年第6期654-659,663,共7页Chinese Journal of Forensic Medicine

基　　金：国家重点研发计划项目(2021YFF0602504);国家自然科学基金重点项目(82130056)。

摘　　要：目的 建立金刚烷胺的死后再分布动物模型,研究其在大鼠体内的死后再分布规律,为金刚烷胺相关案件的法医学鉴定提供实验依据。方法 126只雄性SD大鼠,随机分为3组(L组、M组、H组),按治疗极量、LD50和2LD50灌胃,未死的按照LD50平均死亡时间处死,仰卧位置于20℃条件下,按照0 h、6 h、12 h、24 h、48 h、72 h、96 h采集心血、外周血、心、肝、脾、肺、肾、脑、肌肉、睾丸并检测金刚烷胺的含量。结果 治疗极量的金刚烷胺入体后,在心血、心、肝中的含量在死后6 h前下降随后上升,在脾、肾、脑、肌肉和睾丸组织中的含量在死后48 h前都较为稳定,并于96 h点达到峰值,但在肺脏中的含量随时间呈现逐渐下降的趋势;LD50剂量的金刚烷胺入体后,在各组织中的含量均在死后24 h前有下降趋势,随后上升,在外周血、脾、肾、肌肉中的含量于死后48 h点达到峰值,在心血和睾丸中的含量于72 h点达到峰值,在肝、肺、脑中的含量于96 h点达到峰值;2LD50剂量的金刚烷胺入体后,在心、肝中的含量在死后12 h前有下降趋势,在肺、脑、肌肉中的含量在死后48 h前均有下降趋势,在心脏、肝、脾、肌肉、睾丸中的含量在死后96 h点达到峰值。结论 金刚烷胺中毒死亡大鼠体内存在死后再分布现象,可为金刚烷胺中毒死亡案件的法医学鉴定提供实验依据。Objective To establish an animal model of postmortem redistribution of amantadine,and to study its postmortem redistribution in rats,so as to provide experimental evidence for forensic identification.Methods One hundred and twenty-six male SD rats were randomly divided into 3 groups and subjected to intragastric administration according to the maximum dose of treatment(L),LDso(M)and 2LDso(H).Those who did not die were killed according to the average time of death of LDso.Heart-blood,peripheral blood,heart,liver,spleen,lung,kidney,brain,muscle and testis were collected at 0 h,6 h,12 h,24 h,48 h,72 h and 96 h after death,and amantadine content was detected.Results For the rats in the L group,the concentration of amantadine decreased within 6 h after death and then increased in the heart-blood,heart and liver,unchanged within 48 h and reached the peak at 96 h in the spleen,kidney,brain,muscle and testis,while decreased in the lung.For the rats in the M group,the concentration of amantadine decreased within 24 h after death and then increased in all samples,and it reached the peak at 48 h after death in the peripheral blood,spleen,kidney and muscle tissues,at 72 h after death in the heart-blood and testis,and at 96 h after death in the liver,lung and brain tissues.For the rats in the H group,the concentration of amantadine showed a downward trend within 12 h after death in the heart and liver tissue,showed a downward trend within 48 h after death in the lung,brain and muscle tissue,and reached the peak at 96 h after death in the heart,liver,spleen,muscle and testicle tissues.Conclusion The postmortem redistribution was found in amantadine poisoning dead rats,which could provide experimental evidence for the forensic identification of death cases causedbyamantadinepoisoning.

关 键 词：金刚烷胺 死后再分布 LC-MS/MS 大鼠 

分 类 号：D918.93[政治法律—法学]