Single-molecule RNA capture-assisted droplet digital loop-mediated isothermal amplification for ultrasensitive and rapid detection of infectious pathogens  

作　　者：Liying Jiang Xianghao Lan Linjiao Ren Zhiyuan Jin Xuchen Shan Mingzhu Yang Lingqian Chang 

机构地区：[1]School of Electrical and Information Engineering,Zhengzhou University of Light Industry,450002 Zhengzhou,China [2]Academy for Quantum Science and Technology,Zhengzhou University of Light Industry,450002 Zhengzhou,China [3]Beijing Advanced Innovation Center for Biomedical Engineering,School of Biological Science and Medical Engineering,Beihang University,100191 Beijing,China [4]School of Physics,Beihang University,100191 Beijing,China [5]Beijing Research Institute of Mechanical Equipment,100143 Beijing,China

出　　处：《Microsystems & Nanoengineering》2023年第5期151-160,共10页微系统与纳米工程（英文）

基　　金：supported by the National Natural Science Foundation of China(Nos.81902167,32071407,62003023,32101088,12072010,and 62073299);the National Key Research and Development Program of China(2022YFB3205600);the Beijing Natural Science Foundation(No.7212204);the Project of Central Plains Science and Technology Innovation Leading Talents(No.224200510026).

摘　　要：To minimize and control the transmission of infectious diseases,a sensitive,accurate,rapid,and robust assay strategy for application on-site screening is critical.Here,we report single-molecule RNA capture-assisted digital RT-LAMP(SCADL)for point-of-care testing of infectious diseases.Target RNA was captured and enriched by specific capture probes and oligonucleotide probes conjugated to magnetic beads,replacing laborious RNA extraction.Droplet generation,amplification,and the recording of results are all integrated on a microfluidic chip.In assaying commercial standard samples,quantitative results precisely corresponded to the actual concentration of samples.This method provides a limit of detection of 10 copies mL−1 for the N gene within 1 h,greatly reducing the need for skilled personnel and precision instruments.The ultrasensitivity,specificity,portability,rapidity and user-friendliness make SCADL a competitive candidate for the on-site screening of infectious diseases.

关 键 词：SCAD RECORDING COMPETITIVE 

分 类 号：R511[医药卫生—内科学]