荧光适配体生物传感器的构建及用于检测中药饮片中的啶虫脒  

Fabrication of fluorescent aptamer sensor for the detection of acetamiprid in Chinese prepared slices of crude drugs

作　　者：张红艳[1] 陈淑萍 范申 张淑玲[1] 余宇燕[1] 林舒[1] ZHANG Hongyan;CHEN Shuping;FAN Shen;ZHANG Shuling;YU Yuyan;LIN Shu(Pharmacy College of Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)

机构地区：[1]福建中医药大学药学院,福州350122

出　　处：《分析试验室》2023年第12期1584-1590,共7页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金项目(81703695);福建省自然科学基金面上项目(2021J01921,2022J01359);福建中医药大学校管项目(X2021014-重点,X2020013)资助。

摘　　要：构建了一种基于纳米金(AuNPs)和碳量子点(CQDs)的荧光适配体传感方法,并用于啶虫脒的快速检测。在优化条件下,荧光强度与啶虫脒浓度在20~100μg/mL范围内具有良好的线性关系,相关系数为0.9972,检出限为8.31μg/mL。应用本方法对中药饮片山药和太子参中的啶虫脒进行加标回收实验,回收率为90.8%~105.7%,相对标准偏差(RSD)为0.4%~5.8%。该方法有望用于啶虫脒现场快速定量分析。A fluorescent aptamer biosensor based on gold nanoparticles(AuNPs)and carbon quantum dots(CQDs)for the rapid detection of acetamiprid was fabricated.Under the optimized conditions,there was a good linear relationship between the fluorescence intensity and the acetamiprid concentration in the range of 20-100μg/mL,with a correlation coefficient of 0.9972,and the detection limit was 8.31μg/mL.This method was applied to the spiked recovery experiment of acetamiprid in traditional Chinese medicine slices of yam and Radix Pseudostellariae,the recoveries ranged from 90.8%to 105.7%,and the relative standard deviations(RSDs)were 0.4%~5.8%.This method is expected to be used for the on site rapidly quantitative analysis of acetamiprid.

关 键 词：荧光生物传感器 农药残留 啶虫脒 适配体 

分 类 号：O657.31[理学—分析化学]