基于核酸适体的试纸条检测三磷酸腺苷  

Determination of adenosine triphosphate by aptamer strips

作　　者：任林娇 王朋伟 陈青华 齐汝宾 闫艳霞 张培 秦自瑞 陈高凯 姜利英 REN Linjiao;WANG Pengwei;CHEN Qinghua;QI Rubin;YAN Yanxia;ZHANG Pei;QIN Zirui;CHEN Gaokai;JIANG Liying(College of Electrical and Information,Zhengzhou University of Light Industry,Zhengzhou 450002,China;College of Computer and Communication Engineering,Zhengzhou University of Light Industry,Zhengzhou 450002,China;Academy for Quantum Science and Technology,Zhengzhou University of Light Industry,Zhengzhou 450002,China)

机构地区：[1]郑州轻工业大学电气信息工程学院,郑州450002 [2]郑州轻工业大学计算机与通信工程学院,郑州450002 [3]郑州轻工业大学量子科技研究院,郑州450002

出　　处：《分析试验室》2023年第12期1606-1614,共9页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金项目(62073299,12004348,61801436);中原科技创新领军人才项目(224200510026);河南省高校科技创新团队支持计划(20IRTSTHN017);河南省科技攻关项目(212102210196,212102210278,212102210002)资助。

摘　　要：将核酸适体(Aptamer)的特异性与纳米金颗粒的独特光学性质相结合,制备了一种适用于小分子检测的干式试纸条。该试纸条以修饰功能化核酸适体(PloyT13-Aptamer)的纳米金为识别元件,在控制线(C)上修饰ployA13序列,与识别元件结合以判断试纸条的有效性;在测试线(T)上修饰与Aptamer部分互补的DNA序列,与待测物形成竞争关系,通过T线上纳米金显色的深浅来定性或定量分析待测物浓度。结果表明,优化实验条件下,观察T线颜色变化可实现三磷酸腺苷(ATP)的肉眼定性检测,视觉检出限为10μmol/L。使用Image J软件进行定量分析,试纸条检测范围为10~1000μmol/L,检出限为2.4μmol/L。该试纸条生物传感器可以在10 min内得出检测结果且特异性良好,血清中回收率为104.4%~119.0%,为ATP的现场快速检测提供了一种经济有效的方法。A dry strip for small molecule detection was prepared by combining specific aptamers and gold nanoparticles(AuNPs)with unique optical properties.The ployA13 sequence was modified on the control line(C)and the PloyT13-Aptamer nanogold was used as the recognition element to judge the validity of the strip.The DNA sequence complementary to aptamer was modified on the test line(T)to form a competitive relationship with the object to be tested.The concentrations of the target is capable of being analyzed qualitatively and quantitatively by the color shade of the AuNPs on the T line.The results exhibited that the color change of the T-line can be observed under optimized conditions,and the visual detection limit of ATP was 10μmol/L.The detection range of the strip was 10-1000μmol/L with a detection limit of 2.4μmol/L,when Image J software was used for quantitative analysis.The strip biosensor can produce results within 10 min with excellent specificity,and the serum recovery rates were 104.4%-119.0%,which provides an economical and effective strategy for rapid detection of ATP in relevant fields.

关 键 词：试纸条 核酸适体 纳米金颗粒 三磷酸腺苷 

分 类 号：O657.3[理学—分析化学]