人体呼出气体一氧化氮的气液相化学发光检测  

Gas-liquid phase chemiluminescence detection of human exhaled gas nitric oxide

作　　者：熊桠林 毛娅[2] 黄丹 范力瑞 付大友 谭文渊 张欢 吴雨燃 XIONG Yalin;MAO Ya;HUANG Dan;FAN Lirui;FU Dayou;TAN Wenyuan;ZHANG Huan;WU Yuran(School of Chemical Engineering,Sichuan University of Science and Engineering,Zigong 643000,China;School of Pharmaceutical Products and Environmental Engineering,Sichuan Vocational College of Chemical Technology,Luzhou 646009,China;Luzhou City Emergency Support Center,Luzhou 646000,China)

机构地区：[1]四川轻化工大学化学工程学院,自贡643000 [2]四川化工职业技术学院药品与环境工程学院,泸州646009 [3]泸州市应急保障中心,泸州646000

出　　处：《分析试验室》2023年第12期1644-1648,共5页Chinese Journal of Analysis Laboratory

基　　金：四川轻化工大学研究生创新基金(Y2021012);泸州市科技局重点研发项目(2021-SYF-43)资助。

摘　　要：通过选择KMnO_(4)氧化剂将NO气体氧化为NO_(2)气体,基于鲁米诺(Luminol)化学发光检测NO_(2)气体的体系,采用自主研发的气液相化学发光检测仪,建立了一种化学发光检测呼吸系统疾病中痕量NO气体的方法。研究了KMnO_(4)浓度,pH以及Luminol检测NO_(2)体系中各组分浓度对化学发光强度的影响。结果表明,在KMnO_(4)浓度为0.15 mol/L、pH 1.7时氧化效果最好,在Luminol(5.0 mmol/L)、KOH(50 mmol/L)体系中加入Na_(2)SO_(3)(0.10 mol/L)、乙二醇(3.0%(V/V))能显著增强Luminol体系检测NO_(2)的化学发光信号。最优条件下,在10~250μg/L范围内,NO浓度与发光强度呈现出良好的线性关系。A chemiluminescence method for the detection of trace level of NO gas in respiratory diseases was established by a self-developed gas-liquid chemiluminescence detector,which the main mechanism was based on KMnO_(4)oxidizing NO to NO_(2)and generating chemiluminescence signal.The influence of the concentration of KMnO_(4),pH and Luminol on the chemiluminescence intensity in NO_(2)system were investigated.The oxidation effect was optimal at the KMnO_(4)concentration of 0.15 mol/L and pH value of 1.7.Meanwhile,the additions of Na_(2)SO_(3)(0.10 mol/L)and ethylene glycol(3%)in the Luminol(5.0 mmol/L)and the KOH(50 mmol/L)can significantly enhance the intensity of luminol system.Under the optimal conditions,the NO concentration showed a favorable linear relationship with the luminescence intensity in the range of 10-250μg/L.This method possessed multiple benefits like high accuracy,good stability,easy carrying and green environmental protection.

关 键 词：气液相化学发光 一氧化氮 鲁米诺 呼吸系统疾病 

分 类 号：O659.21[理学—分析化学]