垫料生物质活性炭修饰电极的制备及对多巴胺和尿酸的同步检测  

Preparation and simultaneous detection of dopamine and uric acid by bedding biomaterial activated carbon modified electrode

作　　者：荣成[1,2] 黎伟 李振国 景蕊 郑新宇 黄彪[2] RONG Cheng;LI Wei;LI Zhenguo;JING Rui;ZHENG Xinyu;HUANG Biao(College of Life Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China;College of Material Engineering,Fujian Agriculture and Forestry University,Fuzhou 350002,China)

机构地区：[1]福建农林大学生命科学学院,福州350002 [2]福建农林大学材料工程学院,福州350002

出　　处：《分析试验室》2023年第11期1461-1467,共7页Chinese Journal of Analysis Laboratory

基　　金：福建省自然科学基金(2019J01383);广西民族大学重点实验室开放课题(GXFK2007)资助。

摘　　要：通过碱活化法制备了垫料生物质活性炭(BMAC),元素分析显示除C外,其还含N,S等杂原子。通过扫描电镜(SEM)和拉曼光谱等方法对BMAC进行了表征,结果显示BMAC表面孔道丰富,并呈现一定的石墨化。N^(2)吸-脱附测试显示BMAC的比表面积为408.43 m^(2)/g,平均孔径为3.08 nm。将BMAC复合在玻碳电极(GCE)上制备了BMAC/GCE复合电极。多巴胺(DA)和尿酸(UA)在BMAC/GCE上具有独立的氧化峰,峰电位分别为305 mV和432 mV。在pH 6、BMAC负载量为3μg时,DA和UA在BMAC/GCE的独立氧化峰电流达到最强。在0.5~20μmol/L以及20~200μmol/L范围内,DA,UA的峰电流与其浓度均存在2段线性关系,在BMAC/GCE的检出限均低至0.1μmol/L。BMAC/GCE具有可靠的重复使用性和抗干扰性,其对实际样品中DA和UA的加标回收率分别为98.3%~101.1%和98.8%~104.0%,表明BMAC/GCE具有潜在的应用价值。The bedding biomaterial activated carbon(BMAC)was prepared through the alkali activation method.Element analysis showed that except C element,there are hybrid atoms such as N,S in BMAC.Scanning electron microscopy(SEM)and Raman spectrum were employed to characterize BMAC,and the results showed abundant pores spread all over BMAC with a certain degree of graphitization.The adsorption-desorption of N^(2)revealed the pore size was about 3.08 nm and the specified surface area was about 408.43 m^(2)/g for BMAC.The composite electrode of BMAC/GCE was assembled to detect dopamine(DA)and uric acid(UA)by dispensing some BMAC on the surface of the glass carbon electrode(GCE).The results showed that DA and UA presented independent oxidation peaks on the surface of BMAC/GCE,which reached the maximum at pH 6 and the loading amount of BMAC on GCE of 3μg.The peak currents of DA and UA had two-stage linear relationships with their concentrations in the ranges of 0.5-20μmol/L and 20-200μmol/L,respectively,and the detection limits on BMAC/GCE were all as low as 0.1μmol/L.BMAC/GCE has reliable reusability and anti-interference,and the adding standard recoveries for real DA and UA samples were 98.3%-101.1%and 98.8%-104.0%,respectively,meaning BMAC/GCE has potential application value.

关 键 词：垫料 活性炭 多巴胺 尿酸 检测 

分 类 号：TQ15[化学工程—电化学工业]