不同来源金黄色葡萄球菌对消毒剂的抗性试验观察  

Experimental observation on disinfectant resistance of Staphylococcus aureus from different sources

作　　者：何洁 周元晨 黄珊珊 金园园 劳华均 HE Jie;ZHOU Yuan-chen;HUANG Shan-shan;JIN Yuan-yuan;LAO Hua-jun(Ningbo Joysun Product Testing Service Company,Ningbo Zhejiang315012;Technology Center of Ningbo Customs,China)

机构地区：[1]宁波中盛产品检测有限公司,浙江宁波315012 [2]宁波海关技术中心

出　　处：《中国消毒学杂志》2023年第12期888-890,894,共4页Chinese Journal of Disinfection

基　　金：海关总署科研项目(2021HK195);宁波中盛产品检测有限公司科研项目(2021ZS001)。

摘　　要：目的研究不同来源的金黄色葡萄球菌对消毒剂抗性及筛选相关基因。方法采用基因检测技术和定量杀菌试验方法,检测不同来源金黄色葡萄球菌对常见消毒剂抗性及相关基因。结果在常温下75%乙醇、2000 mg/L季铵盐、5000 mg/L聚六亚甲基双胍分别作用1 min,对悬液内不同来源金黄色葡萄球菌的平均杀灭对数值均>5.00。有效氯1000 mg/L的含氯消毒剂对悬液内4株临床分离株(编号Y7、Y19、Y24、Y13)作用3 min,平均杀灭对数值均>5.00。3000 mg/L三氯羟基二苯醚对4株临床分离株(编号Y7、Y19、Y24、Y13)作用3 min,平均杀灭对数值均<5.00。3株医院感染临床分离株(编号Y7、Y19、Y24)检出耐药基因ermA、TetM、mecA、acc-aph和耐消毒剂基因qacA。结论食源性和医院感染金黄色葡萄球菌对低效消毒剂抗性存在差异,部分医院感染临床分离株检出耐药基因和耐消毒剂基因。Objective To study disinfectant resistance and select related genes of Staphylococcus aureus(S.aureus)from different sources.Methods The gene detection and the quantitative germicidal test were used to detect the resistance of S.aureus to common disinfectants and related genes.Results The killing logarithm values of 75%ethanol,2000 mg/L quaternary ammonium salt,5000 mg/L polyhexamethylene biguanide to S.aureus with 1 min contact time were all>5.00.The average killing logarithm values of 1000 mg/L effective chlorine to 4 clinical strains(No.Y7,Y19,Y24,Y13)with 1 min were all>5.00.The average killing logarithm values of 3000 mg/L trichloro hydroxydiphenyl ether against 4 clinical strains(No.Y7,Y19,Y24,Y13)with 3 min were all<5.00.Drug resistance gene ermA,TetM,mecA,acc-aph and disinfectant resistance gene qacA were detected in 3 clinical strains(No.Y7,Y19,Y24).Conclusion There are different in the resistance of S.aureus to disinfectants between foodborne and hospital sources.Drug resistance genes and disinfectant resistance genes are detected in some clinical strains.

关 键 词：金黄色葡萄球菌 耐药基因 耐消毒剂基因 消毒剂抗性 

分 类 号：R183.32[医药卫生—流行病学] R187.2[医药卫生—公共卫生与预防医学]