miR-140-3p在大鳞副泥鳅雌雄性腺中的差异表达和调控功能的研究  

Differential Expression and Regulatory Function of miR-140-3p in Male and Female Gonads of Paramisgurnus dabryanus

作　　者：吕晓洁 王伟伟[1] 郝瑞荣[1] 刘少贞[1] 陈越 杨琼 李欣欣 谢宇浩 孙睿 LU Xiaojie;WANG Weiwei;HAO Ruirong;LIU Shaozhen;CHEN Yue;YANG Qiong;LI Xinxin;XIE Yuhao;SUN Rui(College of Animal Science,Shanxi Agricultural University,Taigu,Shanxi 030801,China;Qinshui County Modern Agricultural Development Center,Qinshui,Shanxi 048200,China)

机构地区：[1]山西农业大学动物科学学院,山西太谷030801 [2]沁水县现代农业发展中心,山西沁水048200

出　　处：《家畜生态学报》2024年第1期16-21,52,共7页Journal of Domestic Animal Ecology

基　　金：国家自然科学基金资助项目(31600416);山西省自然科学基金资助项目(201601D202080);山西省畜牧“1331重点学科”(J201911306);山西农业大学科技创新基金项目(2016ZZ03)。

摘　　要：miR-140-3p是在不同物种性腺组织中广泛表达的一种miRNA。本研究利用qRT-PCR方法检测miR-140-3p在大鳞副泥鳅性腺中的雌雄表达差异并预测其生物学功能。结果显示:miR-140-3p在大鳞副泥鳅雌雄鱼的性腺组织中均有表达,且精巢中的表达量显著高于卵巢(P<0.01);KEGG pathway分析表明,靶基因CPEB4和GDF9参与卵母细胞成熟分化过程,富集在与性腺发育、繁殖功能相关的孕激素介导卵母细胞成熟通路、卵母细胞减数分裂通路、卵巢类固醇生成通路。荧光定量结果显示CPEB4和GDF9在卵巢中表达量显著高于精巢(P<0.01),且表达量与miR-140-3p的表达呈显著负相关,说明miR-140-3p和CPEB4、GDF9具有潜在的靶标关系。这为进一步探索miR-140-3p在鱼类性腺发育和繁殖过程中的作用奠定了基础。MiR-140-3p is a miRNA widely expressed in gonadal tissues of different species.qRT-PCR was used to detect the expression difference between male and female of miR-140-3p in gonads of Parabgurnus dabryanus,and its biological function was analyzed by predicting related target genes.The results showed that miR-140-3p was expressed in both testis and ovaries,and the expression level of miR-140-3p in testis was significantly higher than that in ovary(P<0.01).KEGG pathway analysis showed that target genes CPEB4 and GDF9 were involved in oocyte maturation and differentiation,and were enriched in progesterone mediated oocyte maturation pathway,oocyte meiosis pathway and ovarian steroid generation pathway,respectively.These pathways were related to gonad development and reproduction.qRT-PCR results showed that the expression of CPEB4 and GDF9 in ovary was significantly higher than that in testis(P<0.01),and the expression level was significantly negatively correlated with expression of miR-140-3p,and these results suggest that miR-140-3p has a potential target relationship with CPEB4 and GDF9.This study provides basic information for future exploration of the role of miR-140-3p in gonad development and reproduction of fish.

关 键 词：大鳞副泥鳅 MiR-140-3p 性腺 

分 类 号：S917.4[农业科学—水产科学] S966.4