丹参素对UVB诱导的皮肤光老化小鼠的保护作用和抗氧化机制研究  

Protective effect and antioxidant mechanism of Danshensu on UVB-induced skin photoaging in mice

作　　者：王安娜 方梦婕 唐超 岳天翔[2] Anna Wang;Mengjie Fang;Chao Tang;Tianxiang Yue(Jiangxi Pharmaceutical School,Nanchang,Jiangxi 330200,China;Jiangxi University of Chinese Medicine,Nanchang,Jiangxi 330004,China)

机构地区：[1]江西省医药学校,江西南昌330020 [2]江西中医药大学,江西南昌330004

出　　处：《日用化学工业（中英文）》2024年第1期65-72,共8页China Surfactant Detergent & Cosmetics

摘　　要：研究丹参素(DSS)对紫外线B(UVB)诱导的皮肤光老化(SP)小鼠的保护作用和抗氧化机制。建立了UVB诱导的SP小鼠模型,使用3个剂量的DSS(20,40和80 mg/(kg·d))治疗小鼠8周,结束后分别测定了各组小鼠的表皮含水量。通过HE染色评价皮肤组织形态,Masson三色染色评价胶原蛋白沉积。按照试剂盒说明测定皮肤组织中氧化应激指标(SOD、CAT、GSH-Px和MDA)和炎症指标(TNF-α和IL-6)水平。通过RT-qPCT和Western blotting检测了皮肤组织中MMP-1、Collagen I、Nrf2、Keap1、HO-1、NF-κB p65和p-NF-κB p65的mRNA或蛋白水平。结果显示,DSS剂量依赖性地提高了UVB诱导的SP小鼠表皮含水量,减轻皮肤损伤,促进胶原形成(P<0.05)。DSS抑制了UVB诱导的SP小鼠皮肤组织中MMP-1的转录和表达,促进了Collagen的转录和表达(P<0.05)。DSS升高了UVB诱导的SP小鼠皮肤组织中SOD、CAT和GSH-Px的水平,降低了MDA的水平(P<0.05)。DSS降低了UVB诱导的SP小鼠皮肤组织中TNF-α和IL-6的水平(P<0.05)。DSS促进了UVB诱导的SP小鼠皮肤组织中Nrf2和HO-1的转录和表达,抑制了Keap1的转录和表达(P<0.05)。DSS抑制了UVB诱导的SP小鼠皮肤组织中p-NF-κB p65的表达(P<0.05)。本研究表明DSS可有效改善UVB诱导的小鼠SP,其机制与Nrf2和NF-κB信号通路有关。The study aims to reveal the protective effect and antioxidant mechanism of Danshensu(DSS)on ultraviolet B(UVB)-induced skin photoaging(SP)in mice.In this study,a UVB-induced SP mouse model was established,and then the mice were treated with 3 doses of DSS(20,40,and 80 mg/(kg·d))for 8 weeks.After the treatment,the water content of the epidermis of the mice in each group was measured respectively.Skin tissue morphology was evaluated by HE staining,and collagen deposition was evaluated by Masson’s trichrome staining.The levels of oxidative stress indicators(SOD,CAT,GSH-Px and MDA)and inflammatory indicators(TNF-αand IL-6)in the skin tissue were determined according to the kit instructions.The mRNA or protein levels of MMP-1,CollagenI,Nrf2,Keap1,HO-1,NF-κB p65 and p-NF-κB p65 in the skin tissue were detected by RT-qPCT or Western blotting.The results show that DSS dose-dependently increases the water content of the epidermis of SP mice induced by UVB,alleviates skin damage,and promotes collagen formation(P<0.05).DSS inhibits the UVB-induced transcription and expression of MMP-1 in the skin tissue of SP mice,and promotes the transcription and expression of Collagen(P<0.05).DSS increases the levels of SOD,CAT and GSH-Px in the skin tissue of SP mice induced by UVB,and decreases the level of MDA(P<0.05).DSS decreases the levels of TNF-αand IL-6 in the skin tissue of SP mice induced by UVB(P<0.05).DSS promotes the transcription and expression of Nrf2 and HO-1 in the skin tissue of SP mice induced by UVB,and inhibits the transcription and expression of Keap1(P<0.05).DSS inhibits the expression of p-NF-κB p65 in the skin tissue of SP mice induced by UVB(P<0.05).In conclusion,this study shows that DSS can effectively improve UVB-induced SP in mice,and its mechanism is related to Nrf2 and NF-κB signaling pathways.

关 键 词：丹参素 紫外线B 皮肤光老化 小鼠 抗氧化 

分 类 号：TQ658[化学工程—精细化工]