蛋白质复合物表达纯化技术的研究进展  

Research Progress in Protein Complex Expression and Purification Technology

作　　者：李思葳 李佳 臧奕 LI Siwei;LI Jia;ZANG Yi(School of Pharmaceutical Science and Technology,Hangzhou Institute for Advanced Study,Chinese Academy of Sciences,Hangzhou 310012,China;Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 201203,China;Lingang Laboratory,Shanghai 200031,China)

机构地区：[1]国科大杭州高等研究院药物科学与技术学院,杭州310012 [2]中国科学院上海药物研究所,上海201203 [3]临港实验室,上海200031

出　　处：《中国细胞生物学学报》2023年第10期1543-1550,共8页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(重大研究计划项目)(批准号:92253306)资助的课题。

摘　　要：蛋白质复合物作为细胞的功能分子,在生物体信号传递及功能调控中发挥重要作用,复合物结构的解析对于研究蛋白间相互作用的分子机制、预测蛋白功能、预测药物靶标以及药物设计都具有重要意义。目前蛋白质复合物的纯化方式主要有三种:纯化获得复合物单一组分后在体外进行组装形成稳定复合体;将复合物全部组分表达至同一宿主中,使复合物在体内发生组装;纯化内源蛋白质复合物。该文归纳综述了当前蛋白质复合物纯化的三种方式,以期为蛋白复合物纯化技术的发展及其他重要蛋白复合物结构的研究提供参考。Protein complex,the functional molecule of cell,appears to play a central role in signal transduction and functional regulation of organism.The structure of protein complex is of great significance for studying the molecular mechanism of protein-protein interaction,predicting protein function,discovering potential drug targets and contributing to drug design.Three methods are mainly used for purifying protein complexes so far.One major technique is to obtain complex components separately and assemble in vitro;another is co-expression of protein components in the same host cell following by assembling in vivo;the third one is purification of endogenous protein complexes,notably through the use of knock-in method.Here,this study summarizes three major techniques for isolating protein complex and anticipates to provide reference for further researches on important protein complex structure.

关 键 词：蛋白表达纯化 蛋白-蛋白相互作用 蛋白质复合物 

分 类 号：Q51[生物学—生物化学]