噬菌体抗细菌生物膜机制及应用策略的研究进展  

Advances in the Mechanism of Phage Resistance to Bacterial Biofilms and Strategies for Its Application

作　　者：杨佩霓 李庆蓉[1] 李江[1] 何薇[1] 和平安 吕梅 杨旭[1] YANG Peini;LI Qingrong;LI Jiang;HE Wei;HE Ping’an;LÜMei;YANG Xu(Department of Clinical Laboratory,the Second Affiliated Hospital of Kunming Medical University,Kunming 650032,China)

机构地区：[1]昆明医科大学第二附属医院检验科,昆明650032

出　　处：《现代检验医学杂志》2024年第1期199-204,共6页Journal of Modern Laboratory Medicine

基　　金：国家自然科学基金(82060669):绿原酸激活细胞自噬抵抗肺炎克雷伯菌感染的分子机制研究;云南省科技厅-基础研究专项-面上项目(202101AT070256):绿原酸通过PI3K/Akt-mTOR调控肺组织天然免疫应答抵御肺炎克雷伯菌侵染的分子机制;云南省教育厅科学研究基金项目(2023Y0641):新型恶唑烷酮类耐药基因optrA在粪肠球菌中的水平传播机制;昆明医科大学第二附属医院内科技计划资助项目(2021yk002):应用MALDI-TOF MS建立一种快速药敏试验测定MIC:基于LREfs耐药性检测;昆明医科大学2023研究生创新基金(2023S315):利奈唑胺耐药基因optrA在临床来源的粪肠球菌间的传播机制研究。

摘　　要：细菌生物膜(bacterial biofilms,BF)是细菌在生物或非生物表面所形成的复杂微生物群落,其形成显著增强了细菌毒力和耐药性,与高比例的慢性细菌感染相关,对人类健康造成严重威胁。传统抗生素和常用消毒剂在清除生物膜方面的能力有限,迫切需要一种有效的新策略治疗细菌生物膜。噬菌体(bacteriophage,phage)作为一类能感染并裂解细菌的病毒,具有较高的安全性和特异度,被认为是治疗细菌生物膜有前景的替代方法。该文综述了噬菌体抗细菌生物膜的作用机制、基于噬菌体及其衍生物在防控细菌生物膜形成的应用策略,为开发高效的噬菌体抗细菌生物膜方法提供新思路。Bacterial biofilms(BF)are complex microbial communities formed by bacteria on living or abiotic surfaces.Their formation significantly enhances bacterial virulence and drug resistance and is associated with a high proportion of chronic bacterial infections,posing a serious threat to human health.The ability of traditional antibiotics and commonly used disinfectants to clear biofilms is limited,and an effective new strategy to treat BF is urgently needed.Bacteriophage,as a kind of virus that can infect and lyse bacteria,has high safety and specificity,and is considered as a promising alternative method for the treatment of BF.In this paper,the mechanism of bacteriophage anti-bacterial biofilm and the application strategies based on bacteriophage and its derivatives in the prevention and control of bacteriophage biofilm formation were reviewed,which provided new ideas for the development of efficient bacteriophage anti-bacterial biofilm methods.

关 键 词：噬菌体 细菌生物膜 噬菌体抗细菌生物膜机制 噬菌体应用 

分 类 号：R446.5[医药卫生—诊断学]