民族药九子连的化学成分及对肝癌细胞的增殖抑制作用  

The ethnic medicine Calanthe fimbriata Franch.:Chemical constituents and their inhibitory activities on the proliferation of HepG2 cells

作　　者：刘杏 张昌龙 陈丽娅 张晓庆 高阳[2] 张海龙[2] LIU Xing;ZHANG Changlong;CHEN Liya;ZHANG Xiaoqing;GAO Yang;ZHANG Hailong(Gaoling Maternal and Child Health Hospital of Xi’an,Xi’an 710200,China;School of Pharmacy,Xi’an Jiaotong University,Xi’an 710061,China)

机构地区：[1]西安市高陵区妇幼保健院,西安710200 [2]西安交通大学药学院,西安710061

出　　处：《西北药学杂志》2024年第1期1-5,共5页Northwest Pharmaceutical Journal

基　　金：国家自然科学基金项目(编号:81673564、81102382);陕西省自然科学基金重点项目(编号:2019JZ-01);陕西省中医药管理局项目(编号:SZYKJCYC-2023-080,2023-ZDYJSY-009,2021-ZZJC002)。

摘　　要：目的研究土家族民族药九子连(Calanthe fimbriata Franch.)的化学成分及其对肝癌细胞的增殖抑制作用。方法用硅胶柱色谱、反相硅胶柱色谱、凝胶柱色谱和高效液相色谱等现代分离技术对九子连进行分离纯化,通过波谱技术结合化合物的理化性质对分离得到的化合物进行结构鉴定;用MTT法检测上述化合物对人肝癌HepG2细胞的增殖抑制作用。结果从土家药九子连中分离得到13个化合物,分别鉴定如下:betulalbuside A(1);roseoside(2);尿苷(3);4-羟基-3-甲氧基苯基β-D-吡喃木糖基(1→6)-O-β-D-吡喃葡萄糖苷(4);arctiiphenolglycoside A(5);salidroside(6);3-methylbutan-1-ol-β-D-glucopyranoside(7);translinalool-3,6-oxide-β-D-glucopyranoside(8);(+)-isolariciresinol(9);腺苷(10);N-反式-对羟基苯乙基香豆酰胺(11);marylau⁃rencinol A(12);callosin(13)。其中化合物13对HepG2细胞具有一定的增殖抑制作用。结论上述13个化合物含1个生物碱、1个木脂素、2个二氢菲、3个芳香苷、2个单萜苷、2个核苷、1个脂肪醇苷和1个环酮苷,均为首次从该植物中分离得到。Objective To study the chemical constituents of Calanthe fimbriata Franch.and their inhibitory activities on the proliferation of HepG2 cells.Methods The methanol extract of C.fimbriata was purified by silica gel column chromatography,ODS column chromatography,Sephadex LH-20 chromatography,high performance liquid chromatography techniques.The structures of the compounds were elucidated by spectroscopic analysis and physicochemical properties.Their inhibitory activities on proliferation of HepG2 cells were tested by MTT method.Results Thirteen compounds were isolated and characterized as follows:betulalbuside A(1);roseoside(2);uridine(3);4-hydroxy-3-methoxyphenyl-β-D-xylopyranosyl(1→6)-O-β-D-glucopyranoside(4);arctiiphenolglycoside A(5);salidroside(6);3-methylbutan-1-ol-β-D-glucopyranoside(7);trans-linalool-3,6-oxide-β-D-glucopyranoside(8);(+)-isolariciresinol(9);adenosine(10);N-trans-p-hydroxyphenethyl coumaramine(11);marylaurencinol A(12);and callosin(13),respectively.And the activity result showed that compound 13 exhibited the inhibitory activity on the proliferation in HepG2 cells.Conclusion The identified compounds mainly contained 1 alkaloid,1 lignin,2 dihydrophenanthrenes,3 aromatic glycosides,2 monoterpene glycosides,2 nucleosides,1 aliphatic alcohol glycoside and 1 cyclic ketone glycoside.And all compounds were isolated from C.fimbriata for the first time.

关 键 词：民族药 土家药 九子连 化学成分 细胞毒作用 

分 类 号：R284.1[医药卫生—中药学]