基于UHPLC-Q-Orbitrap-MS比较不同产地黄芪饮片的成分差异  

Comparison of the difference of chemical compositions in Astragali radix decoction pieces from different origins by UHPLC-Q-Orbitrap-MS

作　　者：刘倩 李格 张静 兰银银 韩福国 范雪梅 郝艳丽 刘清飞 LIU Qian;LI Ge;ZHANG Jing;LAN Yinyin;HAN Fuguo;FAN Xuemei;HAO Yanli;LIU Qingfei(School of Pharmaceutical Sciences,Shaanxi University of Chinese Medicine,Xianyang 712046,China;School of Pharmaceuti-cal Sciences,Tsinghua University,Beijing 100084,China;Beijing Institute of TCM-X,Tsinghua University,Beijing 100084,China)

机构地区：[1]陕西中医药大学药学院,咸阳712046 [2]清华大学药学院,北京100084 [3]清华大学北京市中医药交叉研究所,北京100084

出　　处：《西北药学杂志》2024年第1期12-19,共8页Northwest Pharmaceutical Journal

基　　金：科技部国际合作重点项目(编号:2016YFE011-3700)。

摘　　要：目的探究黄芪的3个重要产地(山西浑源与朔州、甘肃渭源与岷县、陕西子洲)所产黄芪饮片的成分差异,为其基原鉴定与质量研究提供参考依据。方法用超高效液相色谱-四级杆/静电场轨道阱高分辨质谱技术(UHPLC-Q-Orbitrap-MS)测定3个不同产地黄芪饮片的化学成分。以变量投影重要性(VIP)>1、P<0.05为标准,筛选获得其显著差异成分,用SIMCA软件对所得化合物及差异化合物进行聚类分析、偏最小二乘法判别分析(partial least squares discriminant analysis,PLS-DA)。结果4-羟基肉桂酸、胱氨酸、刺芒柄花素等21个化合物在甘肃、陕西、山西3个产地黄芪之间存在显著差异,其中甘肃与山西产地的黄芪差异性成分有1个,山西与陕西产地的黄芪差异成分有11个,甘肃与陕西产地的黄芪差异成分有18个。结论基于UHPLC-QOrbitrap-MS液质联用技术及聚类分析、PLS-DA等成分分析方法所得21个差异性成分,可为不同产地及品种黄芪饮片的来源鉴定及质量控制提供理论依据。Objective To explore the differential components of Astragali radix decoction pieces produced by 3 important production areas(Hunyuan and Shuozhou in Shanxi,Weiyuan and Minxian in Gansu,and Zizhou in Shaanxi)and to provide a reference basis for identifying their origin and quality research.Methods The chemical compositions of Astragali radix decoction pieces from 3 different origins were determined based on UHPLC-Q-Orbitrap-MS liquid-mass spectrometry system.Their significantly differential components were obtained by variable projection importance(VIP)>1 and P<0.05 and the differential compounds were subjected to cluster analysis and partial least squares discriminant analysis(PLS-DA)using SIMCA software.Results 21 compounds,including 4-hydroxycinnamic acid,cystine,prickly mangosteen,were significantly differential between Gansu,Shaanxi and Shanxi,with 1 differential component between Gansu and Shanxi,11 differential components between Shanxi and Shaanxi,and 18 differential components between Gansu and Shaanxi.Conclusion Total of 21 differential components based on the UHPLC-Q-Orbitrap-MS liquid-mass spectrometry system and component analysis methods such as clustering and PLS-DA could provide a theoretical basis for guiding the identification of the medicinal origin and quality control of Astragali radix decoction pieces.

关 键 词：黄芪饮片 不同产地 超高效液相色谱-四级杆/静电场轨道阱高分辨质谱技术 偏最小二乘法判别分析 差异成分 

分 类 号：R284[医药卫生—中药学]