六味能消制剂中非法成分土大黄苷检测方法的建立  

Establishment of the determination method for illegal composition rhaponticin in Liuwei Nengxiao preparations

作　　者：祁晓玲 张炜 杨凤梅 QI Xiaoling;ZHANG Wei;YANG Fengmei(Qinghai Provincial Drug Inspection and Testing Institute,Qinghai Provincial Key Lab of Modernization of Chinese and Tibetan Medi-cine,Key Lab of Chinese and Tibetan Medicine Quality Control of National Medical Products Administration,Xining 810016,China)

机构地区：[1]青海省药品检验检测院,青海省中藏药现代化研究重点实验室,国家药品监督管理局中药(藏药)质量控制重点实验室,西宁810016

出　　处：《西北药学杂志》2024年第1期32-43,共12页Northwest Pharmaceutical Journal

基　　金：2023年中央引导科技发展资金计划项目(编号:2023ZY004)。

摘　　要：目的建立六味能消丸(胶囊、片)中非法成分土大黄苷的检测方法。方法用薄层色谱法及高效液相色谱-二极管阵列检测器法初步筛查样品中非法成分土大黄苷,并用高效液相色谱-串联质谱法对土大黄苷检测阳性结果进一步进行确证。结果在101批次六味能消丸(胶囊、片)中的12批次六味能消丸中检出土大黄苷。结论所建立的方法专属性强,可有效筛查六味能消制剂中是否含有土大黄苷。Objective To establish a method for the determination of illegal composition rhaponticin in Liuwei Nengxiao Pills(Capsules and Tablets).Methods Thin layer chromatography(TLC)method and high performance liquid chromatography(HPLC)method were employed to identify rhaponticin in the samples primarily.Then HPLC-MS method was employed to confirm the rhaponticin in positive samples.Results Among 101 batches of Liuwei Nengxiao Pills(Capsules and Tablets),12 batches containing rhaponticin were detected.Conclusion The established method is specific,and can be used to detect the illegal composition of rhaponticin in Liuwei Nengxiao preparations.

关 键 词：六味能消丸(胶囊、片) 大黄 土大黄苷 薄层色谱法 高效液相色谱法 高效液相色谱法-串联质谱法 

分 类 号：R927.2[医药卫生—药学]