不同血片递送方式对汕头市新生儿葡萄糖-6-磷酸脱氢酶缺乏症检测结果的影响探讨  

Exploration of the Effects of Different Blood Tablet Delivery Methods on the Results of Glucose-6-phosphate Dehydrogenase Deficiency Testing in Newborn Infants in Shantou City

作　　者：邱少汕 Qiu Shaoshan(Department of Laboratory Medicine,Shantou Maternal and Child Health Hospital,Shantou,Guangdong,515000,China)

机构地区：[1]汕头市妇幼保健院检验科,广东汕头515000

出　　处：《黑龙江医学》2024年第2期202-204,共3页Heilongjiang Medical Journal

摘　　要：目的:了解汕头市不同血片递送方式对新生儿葡萄糖-6-磷酸脱氢酶(glucose-6-phosphate dehydrogenase,G6PD)缺乏症检测结果的影响。方法:选取2019年1月1日—2020年12月31日汕头市出生的126 249例新生儿作为研究对象。采集出生72 h并充分哺乳8次以上新生儿的足跟血,制作血滤纸干血片,按递送方式分为派专人递送标本组和普通快递递送标本组。采用荧光定量法测定G6PD,筛查阳性召回后采集末梢血,用G6PD/6PGD比值法进行确诊。结果:派专人递送标本组血片采血时间至收到时间的平均天数普通快递递送标本组明显缩短,差异有统计学意义(u=513,P<0.05)。普通快递递送标本组筛查阳性率明显高于派专人递送标本组,差异有统计学意义(χ^(2)=15.568,P<0.05)。普通快递递送标本组确诊率与派专人递送标本组比较,差异无统计学意义(χ^(2)=1.355,P>0.05)。结论:通过普通快递方式送达的标本G6PD筛查阳性率要明显高于通过派专人送标本方式的标本,通过普通快递方式送达的标本假阳性率高。Objective:To understand the effect of different blood tablet delivery methods on glucose-6-phosphate dehydrogenase(G6PD)deficiency test results in neonates in Shantou City.Methods:126249 neonates born in Shantou City from January 1,2019 to December 31,2020 were selected as the study subjects.Heel blood was collected from newborns who had been born for 72 h and had breastfed more than 8 times,and dried blood samples were prepared on blood filter paper,which was divided into a group of specimens sent by hand and a group of specimens sent by ordinary courier according to the mode of delivery.G6PD was measured by fluorescence quantification,and peripheral blood was collected after screening for positive recall,and the diagnosis was confirmed by the G6PD/6PGD ratio method.Results:The mean number of days from blood film collection time to receipt time in the group sent to deliver specimens by hand was significantly shorter in the group with ordinary courier delivery of specimens,and the difference was statistically significant(u=513,P<0.05).The screening positivity rate was significantly higher in the group with specimens delivered by ordinary courier than in the group with specimens sent by hand,and the difference was statistically significant(χ^(2)=15.568,P<0.05).There was no statistically significant difference in the rate of confirmation of diagnosis in the group of specimens delivered by ordinary courier compared with the group of specimens sent by hand(χ^(2)=1.355,P>0.05).Conclusion:The rate of G6PD screening positivity is significantly higher for specimens delivered by regular courier than for specimens delivered by sending a person to deliver the specimen,and the rate of false positives is high for specimens delivered by regular courier.

关 键 词：新生儿筛查 葡萄糖-6-磷酸脱氢酶 血片递送 

分 类 号：R722[医药卫生—儿科]