人FOXP3基因的生物信息学分析  

Bioinformatic Analysis of the Human FOXP3 Gene

作　　者：陈倩 戴凌燕[1] 李宇琦 李爽 申雪 佟春玉[1] 宋博翠[1] Chen Qian;Dai Lingyan;Li Yuqi;Li Shuang;Shen Xue;Tong Chunyu;Song Bocui(College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319)

机构地区：[1]黑龙江八一农垦大学生命科学技术学院,大庆163319

出　　处：《黑龙江八一农垦大学学报》2023年第6期74-80,87,共8页journal of heilongjiang bayi agricultural university

基　　金：黑龙江省博士后启动基金项目(LBH-Q21158)。

摘　　要：在正常生理和应激生物环境中,叉头转录因子3(FOXP3)是调节性T细胞发育和抑制功能的重要转录因子。试验利用生物信息学在线工具及软件对FOXP3蛋白的理化性质、蛋白晶体结构、翻译后修饰以及其相互作用蛋白等进行了研究。研究发现FOXP3蛋白复合物主要包含NFAT1 DNA结合域和与脱氧核糖核酸结合的FOXP3 FKH结构域,并且通过多种翻译后修饰影响FOXP3蛋白构象和与其互作蛋白的相互作用,最终改变FOXP3蛋白活性以调节Treg抑制特征。研究结果可为今后深入研究FOXP3如何调节Treg抑制的新方法和增强自身免疫性疾病免疫耐受的新疗法提供参考。Forkhead transcription factor 3(FOXP3)played an important role in regulating regulatory T cell development and inhibiting function in normal physiological and stressed biological environments.The physical and chemical properties of FOXP3 protein,its crystal structure,post-translational modification and its interacting proteins were investigated using bioinformatics online tools and software.It was found that FOXP3 protein complex mainly contained the NFAT1 DNA-binding domain and the FOXP3 FKH domain bound to DNA,and affected the conformation of FOXP3 protein and its interaction with proteins through various post-translational modifications,and finally changed FOXP3 protein activity to regulate the inhibition of Treg.The results might provide a reference for further research on how FOXP3 regulates the inhibition of Treg and new therapies to enhance immune tolerance in autoimmune diseases.

关 键 词：TREG细胞 FOXP3基因 免疫耐受 生物信息学分析 

分 类 号：Q811.4[生物学—生物工程]