Calcium-chelating peptides from rabbit bone collagen:characterization,identification and mechanism elucidation  

作　　者：Fuhuan Yuan Yu Fu Liang Ma Hankun Zhu Yong Yu Xin Feng Yi Sun Hongjie Dai Xin Liu Zhengfang Liu Yuhao Zhang 

机构地区：[1]College of Food Science,Southwest University,Chongqing 400715,China [2]Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing,Chongqing 400715,China [3]Key Laboratory of Luminescence Analysis and Molecular Sensing(Southwest University),Ministry of Education,Chongqing 400715,China [4]Angel Yeast Co.,Ltd.,Yichang 443003,China

出　　处：《Food Science and Human Wellness》2024年第3期1485-1493,共9页食品科学与人类健康（英文）

基　　金：granted by the National Key R&D Program of China (2021YFD21001005);National Natural Science Foundation of China (31972102,32101980);Special key project of Chongqing technology innovation and application development (cstc2021jscx-cylhX0014);Chongqing Technology Innovation and Application Development Special Project (cstc2021jscx-tpyzxX0014)。

摘　　要：This study aimed to characterize and identify calcium-chelating peptides from rabbit bone collagen and explore the underlying chelating mechanism.Collagen peptides and calcium were extracted from rabbit bone by instant ejection steam explosion(ICSE)combined with enzymatic hydrolysis,followed by chelation reaction to prepare rabbit bone peptide-calcium chelate(RBCP-Ca).The chelating sites were further analyzed by liquid chromatography-tandem mass(LC-MS/MS)spectrometry while the chelating mechanism and binding modes were investigated.The structural characterization revealed that RBCP successfully chelated with calcium ions.Furthermore,LC-MS/MS analysis indicated that the binding sites included both acidic amino acids(Asp and Glu)and basic amino acids(Lys and Arg),Interestingly,three binding modes,namely Inter-Linking,Loop-Linking and Mono-Linking were for the first time found,while Inter-Linking mode accounted for the highest proportion(75.1%),suggesting that chelation of calcium ions frequently occurred between two peptides.Overall,this study provides a theoretical basis for the elucidation of chelation mechanism of calcium-chelating peptides.

关 键 词：Rabbit bone Collagen peptide Peptide-calcium chelate Chelation mechanism Liquid chromatography-tandem mass(LC-MS/MS) 

分 类 号：TS251.94[轻工技术与工程—农产品加工及贮藏工程]