Stability and transepithelial transport of oligopeptide(KRQKYD)with hepatocyte-protective activity from Jinhua ham in human intestinal Caco-2 monolayer cells  

作　　者：Wen Nie Feiran Xu Kai Zhou Jieying Deng Ying Wang Baocai Xu 

机构地区：[1]Engineering Research Center of Bio-process,Ministry of Education,School of Food and Biological Engineering,Hefei University of Technology,Hefei 230601,China [2]School of Biological and Food Engineering,Chuzhou University,Chuzhou 239001,China

出　　处：《Food Science and Human Wellness》2024年第3期1503-1512,共10页食品科学与人类健康（英文）

基　　金：supported by the Major special project of Anhui Province (2021d06050001);the Major Science and Technology Project of Anhui Province (201903b06020004);the Natural Science Foundation of Anhui Province (2308085QC115);the Special Fund for Anhui Province Agricultural Products Processing Industry Technology System (340000211260001000420)。

摘　　要：The study evaluated the stability of an oligopeptide(Lys-Arg-Gln-Lys-Tyr-Asp,KRQKYD)and its transport mechanism by simulating gastrointestinal digestion and a model of human intestinal Caco-2 monolayer cells in vitro.In this study,the effects of environmental factors(temperature,pH and NaCl concentration)and simulated gastrointestinal digestion on the stability of KRQKYD were evaluated by indicators of the levels of alanine transaminase(ALT),aspartate transaminase(AST)and malondialdehyde(MDA)in an alcoholinduced hepatocyte injury model.The results showed that KRQKYD still maintained satisfactory hepatocyteprotective activity after treatment with different temperatures(20-80℃),pH(3.0-9.0),NaCl concentration(1%-7%)and simulated gastrointestinal digestion,which indicated that KRQKYD showed good stability to environmental factors and simulated gastrointestinal digestion.Furthermore,the intact KRQKYD could be absorbed in a model of Caco-2 monolayer cells with a P_(app)value of(9.70±0.53)×10^(-7)cm/s.Pretreatment with an energy inhibitor(sodium azide),a competitive peptide transporter inhibitor(Gly-Pro)and a transcytosis inhibitor wortmannin did not decrease the level of transepithelial KRQKYD transport,indicating that the transport mechanism of KRQKYD was not associated with energy dependent,vector mediated and endocytosis.The tight junction disruptor cytochalasin D significantly increased the level of transepithelial KRQKYD transport(P<0.05),suggesting that intact KRQKYD was absorbed by paracellular transport.

关 键 词：Jinhua ham KRQKYD(Lys-Arg-Gln-Lys-Tyr-Asp) Environmental stability Gastrointestinal digestive Transport mechanism 

分 类 号：TS251.5[轻工技术与工程—农产品加工及贮藏工程]