Effect of simulated gastrointestinal digestion on antioxidant,and anti-inflammatory activities of bioactive peptides generated in sausages fermented with Staphylococcus simulans QB7  

作　　者：Hongying Li Hongbing Fan Zihan Wang Qiujin Zhu Jianping Wu 

机构地区：[1]Laboratory of Animal Genetics,Breeding and Reproduction in the Plateau Mountainous Region,Ministry of Education,College of Life Sciences,Guizhou University,Guiyang 550025,China [2]Department of Agricultural,Food and Nutritional Science,University of Alberta,Alberta T6G 2P5,Canada [3]Department of Animal and Food Sciences,University of Kentucky,Lexington 40546,United States [4]School of Liquor and Food Engineering,Guizhou University,Guiyang 550025,China

出　　处：《Food Science and Human Wellness》2024年第3期1662-1671,共10页食品科学与人类健康（英文）

基　　金：financially supported by the National Natural Science Foundation of China (32272359);Natural Science and Engineering Research Council of Canada (NSERC,RGPIN-2018-04680);the scholarship from the China Scholarship Council (202106670005)。

摘　　要：Dry-fermented sausages are a good source of bioactive peptides,whose stability against gastrointestinal(GI)digestion determines their bioaccessibility.This study focused on evaluating the effect of peptide extracts from sausages fermented with Staphylococcus simulans QB7 during in vitro simulated GI digestion,including peptide profiles and antioxidant and anti-inflammatory activities.Peptides present in sausages were degraded during digestion,with molecular weight reduced from>12 kDa to<1.5 kDa.Besides,the content of amino acids increased from 381.15 to 527.07 mg/g,especially tyrosine being found only after GI digestion.The anti-inflammatory activities were increased after GI digestion,however,the changes in antioxidant activities were the opposite.A total number of 255,252 and 386 peptide sequences were identified in undigested,peptic-digested and GI-digested samples,respectively.PeptideRanker,BIOPEP-UWM and admetSAR were used to further predict the functional properties and intestinal absorption of the identified peptide sequences from GI digestion.Finally,18 peptides were discovered to possess either antioxidant or anti-inflammatory capacities.

关 键 词：Gastrointestinal digestion SAUSAGES BIOACCESSIBILITY Anti-inflammatory activities 

分 类 号：TS251.65[轻工技术与工程—农产品加工及贮藏工程]