Alleviatory effect of isoquercetin on benign prostatic hyperplasia via IGF-1/PI3K/Akt/mTOR pathway  

作　　者：Young-Jin Choi Meiqi Fan Nishala Erandi Wedamulla Yujiao Tang Eun-Kyung Kim 

机构地区：[1]Department of Food Science and Nutrition,Dong-A University,Busan 49315,Republic of Korea [2]Center for Silver-targeted Biomaterials,Brain Busan 21 Plus program,Dong-A University,Busan 49315,Republic of Korea [3]Department of Health Sciences,the Graduate School of Dong-A University,Busan 49315,Republic of Korea [4]Division of Food Bioscience,College of Biomedical and Health Sciences,Konkuk University,Chungju 27478,Republic of Korea [5]Department of Export Agriculture,Faculty of Animal Science and Export Agriculture,Uva Wellassa University,Badulla 90000,Sri Lanka [6]School of Bio-Science and Food Engineering,Changchun University of Science and Technology,Changchun 130600,China [7]Nutritional Education Major,Graduate School of Education,Dong-A University,Busan 49315,Republic of Korea [8]Nutrinomics Lab.Co.,Ltd.,Busan 49315,Republic of Korea

出　　处：《Food Science and Human Wellness》2024年第3期1698-1710,共13页食品科学与人类健康（英文）

基　　金：supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF)funded by the Ministry of Education,Science and Technology (NRF2020R1A2C1014798 to E-K Kim)。

摘　　要：We evaluated the effect of isoquercetin(quercetin-O-3-glucoside-quercetin,IQ)as a functional component of Abeliophyllum disistichum Nakai ethanol extract(ADLE)on prostate cell proliferation and apoptosis and its effects on the IGF-1/PI3K/Akt/mTOR pathway in benign prostatic hyperplasia(BPH).Metabolites in ADLE were analyzed using UHPLC-qTOF-MS and HPLC.IQ was orally administered(1 or 10 mg/kg)to a testosterone propionate-induced BPH rat model,and its effects on the prostate weight were evaluated.The effect of IQ on androgen receptor(AR)signaling was analyzed in LNCaP cells.Whether IGF-1 and IQ affect the IGF-1/PI3K/Akt/mTOR pathway in BPH-1 cells was also examined.The metabolites in ADLE were identified and quantified,which confirmed that ADLE contained abundant IQ(20.88 mg/g).IQ significantly reduced the prostate size in a concentration-dependent manner in a BPH rat model,and significantly decreased the expression of AR signaling factors in the rat prostate tissue and LNCaP cells in a concentration-dependent manner.IQ also inhibited the PI3K/AKT/mTOR pathway activated by IGF-1 treatment in BPH-1 cells.In BPH-1 cells,IQ led to G0/G1 arrest and suppressed the expression of proliferation factors while inducing apoptosis.Thus,IQ shows potential for use as a pharmaceutical and nutraceutical for BPH.

关 键 词：ISOQUERCETIN Benign prostatic hyperplasia Androgen receptor signaling PI3K/Akt/mtor pathway 

分 类 号：R285[医药卫生—中药学]