小鼠亚慢性伏马毒素B_(1)中毒对血液生化指标的影响及组织病理变化的观察  

Effects of Subchronic Fumonisin B_(1)Poisoning on Blood Biochemical Indexes and Observation of Histopathological Lesions in Mice

作　　者：李若彬 朱庆阁 黎晓雯 王凯 刘阳 曹嫦妤 LI Ruo-bin;ZHU Qing-ge;LI Xiao-wen;WANG Kai;LIU Yang;CAO Chang-yu(College of Life Science and Engineering,Foshan University/Foshan University Veterinary Teaching Hospital,Foshan,Guangdong,528231,China;School of Food Science and Engineering,Foshan University/National Technical Center(Foshan)for Quality Control of Famous and Special Agricultural Products(CAQS-GAP-KZZX043)/Guangdong Key Laboratory of Food Intelligent Manufacturing,Foshan,Guangdong,528231,China)

机构地区：[1]佛山科学技术学院生命科学与工程学院/佛山科学技术学院附属教学动物医院,广东佛山528231 [2]佛山科学技术学院食品科学与工程学院/全国名特优新农产品全程质量控制技术佛山中心(CAQS-GAP-KZZX043)/广东省食品智能制造重点实验室,广东佛山528231

出　　处：《动物医学进展》2024年第1期60-66,共7页Progress In Veterinary Medicine

基　　金：国家重点研发计划(2022YFE0139500);广东省基础与应用基础研究基金(2022B1515130003)。

摘　　要：为探究小鼠伏马毒素B_(1)(FB_(1))亚慢性中毒对血液生化指标和组织病理学的影响,选用40只雄性昆明小鼠,通过饲料添加方式建立小鼠FB_(1)的亚慢性中毒模型,分别为对照组(标准基础饲料,C组)和伏马毒素组(5 mg/kg FB_(1)混合基础饲料,FB_(1)组),连续饲喂42 d。每天称重,于试验第21天和第42天取小鼠各器官称重,计算脏器比;测定血液生化指标;HE染色观察肝脏等12种脏器病理组织学变化。结果表明,与C组相比,21 d和42 d中,FB_(1)组肝脏等器官系数显著下降;FB_(1)组血清ALT等含量显著升高,UA等含量显著降低;血常规指标明显异常;病理组织学结果显示,FB_(1)组中肝脏等12种脏器存在一定病理损伤。提示5 mg/kg FB_(1)染毒能够影响小鼠的血液生化指标,且对小鼠各组织器官造成一定损伤。In order to explore the effects of subchronic poisoning of fumonisin B_(1)(FB_(1))in mice on blood biochemical indicators and histopathology,40 male Kunming mice were selected for the experiment,and a subchronic poisoning model of FB_(1)was established by adding feed.The control group(standard basic feed,C group)and fumonisin group(5 mg/kg FB_(1)mixed basic feed,FB_(1)group)were continuously fed for 42 d.Daily weighing,the organs of mice were weighed in the 21 d and 42 d of the experiment to calculate the organ ratio;blood biochemical indicators were measured;HE staining was used to observe the histopathological lesions of liver and other 12 organs.The results showed that,compared with C group,in 21 d and 42 d,the coefficients of organs such as the liver in group FB_(1)decreased significantly;the content of serum ALT in group FB_(1)increased significantly,and the content of UA decreased significantly;blood routine indicators were significantly abnormal;histopathological results showed that there was certain pathological damage to liver and other 12 organs in FB_(1)group.It is suggested that 5 mg/kg FB_(1)infection can affect the blood biochemical indicators of mice and cause certain damage to various tissues and organs of mice.

关 键 词：伏马毒素B_(1) 亚慢性中毒 血液生化 组织损伤 小鼠 

分 类 号：S859.81[农业科学—临床兽医学] S858.91[农业科学—兽医学]