鸡滑液囊支原体的分离鉴定及药敏试验  

Isolation,Identification and Drug Sensitivity Test of Mycoplasmal synoviae in Chicken

作　　者：殷梦玉 张腾飞[1] 张文婷 胡巧 卢琴[1] 郭云清 罗青平[1,2] YIN Meng-yu;ZHANG Teng-fei;ZHANG Wen-ting;HU Qiao;LU Qin;GUO Yun-qing;LUO Qing-ping(The Ministry of Agriculture and Rural Affairs of the Ministry of Agriculture and Rural Affairs of the Key Laboratory of the Prevention and Control of Animal Bacterial Diseases,the Hubei Provincial Key Laboratory of Livestock and Poultry Pathogenic Microbiology,the Institute of Animal Husbandry and Veterinary Medicine of the Hubei Academy of Agricultural Sciences,Wuhan,Hubei,430064,China;Hubei Hongshan Laboratory,Wuhan,Hubei,430064,China)

机构地区：[1]湖北省农业科学院畜牧兽医研究所/农业农村部畜禽细菌病防治制剂创制重点实验室/畜禽病原微生物学湖北省重点实验室,湖北武汉430064 [2]湖北洪山实验室,湖北武汉430064

出　　处：《动物医学进展》2024年第1期130-135,共6页Progress In Veterinary Medicine

基　　金：“十四五”国家重点研发计划项目(2022YFD1800602);湖北省农业科技创重大科技研发专项项目(2020-620-000-002-04,2020-620-000-002-01);国家肉鸡产业技术体系项目(CARS-41);湖北省科技重大专项项目(2020ABA016)。

摘　　要：为弄清湖北省某蛋鸡场180日龄鸡群出现呼吸道症状和产蛋率下降的病原,采集病料进行病原的分离鉴定与药敏试验,用形态学观察方法和分子生物学方法对分离株进行了形态学观察和16S rRNA基因鉴定,并用鸡胚感染试验对分离株进行致病性试验。结果显示,病原分离鉴定结果仅滑液囊支原体阳性,镜检观察到典型的“煎蛋样”菌落;16S rRNA序列与已报道的滑液囊支原体的具有高度同源性;鸡胚感染试验显示,分离株导致鸡胚发育迟缓,胚体弱小,从弱小胚体内可再次分离出滑液囊支原体;药敏试验结果显示滑液囊支原体分离株对泰妙菌素、四环素和盐酸克林霉素敏感。结果表明,从产蛋下降的鸡群中成功分离到1株滑液囊支原体,为临床该病的防治提供了科学依据。In order to confirm the cause of respiratory symptoms and decreased egg production rate in a laying hen farm in Hubei province,the article isolated and cultured the pathogen from the diseased materials,and conducted morphological observations on the isolates using morphological observation methods and molecular biology methods and 16S rRNA gene identification,and the pathogenicity of the isolates was studied using chicken embryo infection test.The results showed that the pathogen isolation and identification results were only positive for Mycoplasma synoviae,and typical"Omelet-like"colonies were observed under microscope;the 16S rRNA sequence was highly homologous to the reported Mycoplasma synoviae;the drug susceptibility results showed that Mycoplasma synovialis was positive.Mycoplasma synoviae isolates are sensitive to tiamulin,tetracycline and clindamycin hydrochloride;chicken embryo infection tests show that the isolate causes delayed development of chicken embryos and weak embryo bodies.Mycoplasma synoviae can be isolated again from the weak embryos.This article successfully isolated a strain of Mycoplasma synoviae from chickens with reduced egg production,providing a theoretical basis for the clinical prevention and treatment of this disease.

关 键 词：鸡 滑液囊支原体 分离鉴定 药敏试验 

分 类 号：S852.62[农业科学—基础兽医学] S858.31[农业科学—兽医学]