基于UPLC-QTOF-MS代谢组学技术的黄苦荞和黑苦荞茶汤差异成分比较  

Comparison of Different Components of Yellow Buckwheat and Black Buckwheat Tea Broth Basedon UPLC-QTOF-MS Metabolomics Technology

作　　者：赵驰 李青 董玲[1] 张凤菊 李治华[1] 阿木布哈 ZHAO Chi;LI Qing;DONG Ling;ZHANG Fengju;LI Zhihua;Amubuha(Institute of Agro-Products Processing of Sichuan Academy of Agricultural Sciences,Chengdu 610066,China;Ganluo Bureau of Education,Sports,Science and Technology,Ganluo 616850,China)

机构地区：[1]四川省农业科学院农产品加工研究所,四川成都610066 [2]甘洛县教育体育和科学技术局,四川甘洛616850

出　　处：《现代食品》2023年第23期179-183,195,共6页Modern Food

基　　金：四川省科技厅科技支撑计划项目(2022YFS0594)。

摘　　要：本研究以黄苦荞和黑苦荞茶汤为研究对象,采用UPLC-QTOF-MS代谢组学技术系统分析两者之间代谢成分的差异。结果显示,茶汤样品在正离子和负离子模式下分别鉴定得到139和103种物质。基于PLS-DA模型分析,发现黄苦荞和黑苦荞茶汤在代谢水平上存在显著差异,并通过VIP值筛选出两者之间的差异代谢物104个。此外,KEGG通路富集分析表明,这些差异代谢物富集在氨基酰-tRNA的生物合成,不饱和脂肪酸的生物合成,半乳糖代谢和木质素、亚木质素和蜡的生物合成等多条代谢通路上。本研究可为明晰黑苦荞和黄苦荞功能成分的差异提供参考。In this study,yellow buckwheat and black buckwheat tea broth were used as research objects,and UPLCQTOF-MS metabolomics technology was used to systematically analyze the differences in metabolic components between the two.The results showed that 139 and 103 substances were identified from the tea broth samples in positive and negative ion modes,respectively.Based on the PLS-DA model analysis,it was found that yellow buckwheat and black buckwheat tea broths differed significantly in metabolic levels,and 104 differential metabolites between the two were screened by VIP values.In addition,KEGG pathway enrichment analysis showed that these differential metabolites were enriched in multiple metabolic pathways,such as aminoacyl-tRNA biosynthesis,unsaturated fatty acid biosynthesis,galactose metabolism and biosynthesis of lignin,suberin and wax.This study can provide a reference for clarifying the differences in functional components between black tartary buckwheat and yellow tartary buckwheat.

关 键 词：黑苦荞茶 黄苦荞茶 代谢组 KEGG富集分析 

分 类 号：O657.63[理学—分析化学]