短刺小克银汉霉AS 3.970对白鲜碱的微生物转化  

Microbial transformation of dictamnine by Cunninghamella blakesleana AS 3.970

作　　者：张金凤 张萌[1] 徐嘉新 陈羽[1] 王淼[1] ZHANG Jinfeng;ZHANG Meng;XU Jiaxin;CHEN Yu;WANG Miao(School of Life Science and Biopharmaceutics,Shenyang Pharmaceutical University,Shenyang 110016,China)

机构地区：[1]沈阳药科大学生命科学与生物制药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2023年第12期1614-1620,共7页Journal of Shenyang Pharmaceutical University

基　　金：国家自然科学基金面上项目(81973284);辽宁省教育厅面上项目(LJKZ0944);辽宁省大学生创新计划项目(S202110163051)。

摘　　要：目的采用短刺小克银汉霉AS 3.970(C.blakesleana AS 3.970)对白鲜碱展开微生物转化研究。方法利用30株丝状真菌对白鲜碱进行微生物转化,筛选出转化效果好的菌株;然后在该菌株微生物转化试验中考察接种量、转化温度、培养基pH值、转化时间等不同因素对白鲜碱微生物转化作用的影响,选择出最优的转化条件;在最优转化条件下,规模制备白鲜碱的微生物转化产物并进行结构鉴定。结果短刺小克银汉霉AS 3.970对白鲜碱的微生物转化效果好,在微生物转化研究中发现白鲜碱转化的最优条件是转化温度为28℃,培养基初始pH值为7.0,最适接种量5%(体积分数),转化时间为7 d;通过分离纯化和结构鉴定,最终得到两个主要转化产物,分别是4-甲氧基-2,3-二氢呋喃喹啉与3-(2-羟乙基)-4-甲氧基喹啉酮。结论通过微生物转化技术可以对白鲜碱进行微生物结构修饰。Objective To study the microbial transformation of dictamnine by Cunninghamella blakesleana AS 3.970(C.blakesleana AS 3.970).Methods 30 filamentous fungi were used to transform dictamnine, and the strains with good transformation effect were screened;The effects of different factors such as inoculation amount, transformation temperature, pH value of culture medium and transformation time on the microbial transformation were investigated;The metabolites were isolated and identified with the optimal transformation conditions.Results The experimental results showed that C.Blakesleana AS 3.970 had a good microbial transformation effect on dictamnine.When the transformation temperature was 28 ℃,the pH value of the culture medium was 7.0,the inoculation amount was 5%,and the transformation time was 7 days, the microbial transformation effect was the best.Two dictamnine transformation metabolites were isolated, and they were identified as 4-methoxy-2,3-dihydro-furoquinoline and 4-methoxy-3-(2-hydroxy-ethyl)-2-oxo-1,2-dihydroquinolinone.Conclusion Microbial transformation could be used to modify the structure of dictamnine.

关 键 词：白鲜碱 短刺小克银汉霉 微生物转化 

分 类 号：R965[医药卫生—药理学]