接种丛枝菌根菌对灰木莲幼苗生长抗旱能力的影响  

Effect of Mycorrhizal Inoculation on Drought Resistance of Magnoliaceae glanca Seedlings

作　　者：曾键鸿 邓海勇 张志光 CENG Jian-hong;DENG Hai-yong;ZHANG Zhi-guang(Huaiji Nature Reserve Administration Center,Huaiji,Guangdong 526400;Huaiji Forestry Bureau,Huaiji,Guangdong 526400;College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou,Guangdong 510000)

机构地区：[1]怀集县自然保护地管理中心 [2]怀集县林业局,广东怀集526400 [3]华南农业大学林学与风景园林学院,广州510000

出　　处：《陕西林业科技》2023年第5期1-5,共5页Shaanxi Forest Science and Technology

基　　金：广东省重点领域研发计划项目(2020B020215012)。

摘　　要：灰木莲抗旱性差,是我国特有濒危保护植物。为实现灰木莲高效栽培,研究应用菌根菌接种技术,提升其抗旱能力意义重大。本试验以灰木莲幼苗为试验材料,利用菌根接种方法,分析了菌根化苗与非菌根化苗在PEG模拟干旱胁迫下的生长与生理差异。结果表明:在4%~12%PEG 6000处理下,菌根与非菌根化幼苗存活率、生物量、叶片水分亏缺、光合参数及抗氧化性均差异显著。其中,在任一浓度PEG处理下,与非菌根化苗相比,菌根化幼苗存活率、生物量增量增大,叶片相对水分亏缺率减小,叶片净光合速率(Pn)、水分利用效率(WUE)、胞间二氧化碳浓度(Ci)、叶绿素含量、丙二醛(MDA)含量降低,蒸腾速率(Tr)、气孔导度(Gs)及过氧化物酶(POD)、过氧化氢酶(CAT)活性增加。表明接种菌根菌,灰木莲幼苗生长抗旱能力明显改善,建议今后可通过菌根苗培育实现灰木莲的科学栽培。Magnoliaceae glance,an endangered plant endemic to China,has poor drought resistance.In order to achieve efficient cultivation of M.glanca,it is of great significance to explore its application potential in improving the drought resistance through mycorrhizal inoculation technology.In this experiment,the seedlings of M.glanca were used as experimental materials,and the differences in growth and physiology between mycorrhizal seedlings and non-mycorrhizal seedlings under PEG simulated drought stress were analyzed.The results showed that the survival rate,biomass,leaf water deficit,photosynthetic parameters and antioxidant activity of mycorrhizal and non-mycorrhizal seedlings were significantly different under 4%~12%PEG 6000 treatment.Among them,any concentration of PEG treatment compared with non-mycorrhizal seedlings,has the survival rate and biomass increment of mycorrhizal seedlings increased,the relative water deficit rate of leaves decreased,and the net photosynthetic rate(Pn),water use efficiency(WUE),intercellular carbon dioxide concentration(Ci),chlorophyll content and malondialdehyde(MDA)content of leaves decreased,and the transpiration rate(Tr),stomatal conductance(Gs)and peroxidase(POD),catalase(CAT)activity increased.The research indicated that the drought resistance of M.glanca seedlings was significantly improved by mycorrhizal inoculation.It is recommended that the scientific cultivation of M.glanca can be realized by mycorrhizal seedling cultivation in the future.

关 键 词：灰木莲 抗旱性 丛枝菌根 高效栽培 

分 类 号：Q949.747.1[生物学—植物学]