经颅重复针刺对血管性痴呆模型大鼠认知功能及海马突触超微结构的影响  

Effects of repetitive transcranial acupuncture on cognitive function and hippocampal synaptic ultrastructure in rats with vascular dementia

作　　者：于国强 孙婧妍[2] 关莹 唐祎周[1] 杨添淞 石光煜[3] 冯秋菊[1] 曹燚 吴壮[1] 张良[1] 李红伟[4] YU Guoqiang;SUN Jingyan;GUAN Ying;TANG Yizhou;YANG Tiansong;SHI Guangyu;FENG Qiuju;Cao Yi;WU Zhuang;ZHANG Liang;LI Hongwei(The Second Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine,Harbin 150001,China;Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,China;The First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,China;Sun Shen-tian TCM Studio,Harbin 150006,China)

机构地区：[1]黑龙江中医药大学附属第二医院,哈尔滨150001 [2]黑龙江中医药大学,哈尔滨150040 [3]黑龙江中医药大学附属第一医院,哈尔滨150040 [4]孙申田名老中医工作室,哈尔滨150006

出　　处：《上海针灸杂志》2024年第1期89-95,共7页Shanghai Journal of Acupuncture and Moxibustion

基　　金：国家自然科学基金面上项目(82074539);黑龙江省卫生健康委科研项目(20211212010322);孙申田青年人才基金(2020KY-12);黑龙江省国医大师学术思想传承科研课题项目(GY2022-19)。

摘　　要：目的 观察经颅重复针刺对血管性痴呆(vascular dementia, VaD)大鼠认知功能及海马CA1区突触素(synaptophysin, SYN)、微管相关蛋白-2(microtubule associated protein-2, MAP-2)表达的影响。方法 将56只SD大鼠随机分为空白组(14只)和造模组(42只)。造模组建立VaD模型,将造模成功大鼠随机分为模型组(14只)、常规针刺组(14只)和经颅重复针刺刺激(repetitive transcranial acupuncture stimulation, rTAS)组(14只)。造模成功后第2天,rTAS组采用经颅重复针刺;常规针刺组仅给予常规针刺手法;模型组与空白组给予以同等条件抓取及固定,不予针刺。采用水迷宫评价大鼠学习记忆能力,采用旷场实验评价大鼠探索行为和自主活动能力,免疫印迹法检测海马CA1区SYP、MAP-2蛋白表达水平,透射电镜观察海马组织中突触超微结构。结果与空白组比较,模型组逃避潜伏时间明显增加(P<0.05),穿越平台次数明显减少(P<0.05);与模型组比较,常规针刺组与rTAS组逃避潜伏时间明显减少(P<0.05),穿越平台次数明显增加(P<0.05);与常规针刺组比较,rTAS组逃避潜伏时间与穿越平台次数改变不明显(P>0.05)。与空白组比较,模型组穿越格子数与后肢站立数明显减少(P<0.05);与模型组比较,常规针刺组与rTAS组穿越格子数与后肢站立数明显增加(P<0.05);与常规针刺组比较,rTAS组穿越格子数与后肢站立数明显增加(P<0.05)。与空白组比较,模型组SYP、MAP-2表达水平显著降低(P<0.05);与模型组比较,常规针刺组与rTAS组SYP、MAP-2表达水平显著增高(P<0.05);与常规针刺组比较,rTAS组SYP、MAP-2表达水平显著增高(P<0.05)。电镜下,模型组突触结构欠清晰,突触间隙模糊不清,突触小泡较少,突触前致密物质减少,胞浆内细胞器稀疏,线粒体变形肿大明显;而rTAS组突触结构较完整,突触间隙、突触前后膜分界清晰,突触前膜内囊泡较多,突触后膜均匀增厚,周边线粒体丰富、结构完�Objective To observe the effects of repetitive transcranial acupuncture on the cognitive function and the expression of synaptophysin(SYN)and microtubule associated protein-2(MAP-2)in hippocampal CA1 region of vascular dementia(VaD)rats.Method Fifty-six SD rats were randomly divided into a blank control group(14 rats)and a model group(42 rats).VaD model was established in the model group,and the successful model rats were randomly divided into a model group(14 rats),a conventional acupuncture group(14 rats)and a repetitive transcranial acupuncture stimulation(rTAS)group(14 rats).On the second day after successful modeling,the rTAS group was treated with repetitive transcranial acupuncture.The rats in the conventional acupuncture group only received conventional acupuncture manipulation.The rats in the model group and the blank control group were grasped and fixed under the same conditions without acupuncture.Water maze was used to evaluate the learning and memory ability of rats,and open field test was used to evaluate the exploratory behavior and autonomous activity ability of rats.Western blot was used to detect the expression of SYP and MAP-2 protein in the hippocampal CA1 region,and transmission electron microscope was used to observe the synaptic ultrastructure of hippocampus.Result Compared with the blank control group,the escape latency time of the model group was significantly increased(P<0.05),and the frequency of crossing the platform was significantly reduced(P<0.05).Compared with the model group,the conventional acupuncture group and the rTAS group had a significant reduction in the escape latency time(P<0.05)and a significant increase in the number of platform crossings(P<0.05).Compared with the routine acupuncture group,the rTAS group had no significant changes in the escape latency time and the number of platform crossings(P>0.05).Compared with the blank control group,the number of crossing grids and the number of hind limb standing in the model group were significantly reduced(P<0.05).Compared wit

关 键 词：针刺疗法 经颅重复针刺 痴呆 血管性 突触 海马 认知功能 大鼠 

分 类 号：R2-03[医药卫生—中医学]