外源水杨酸对浙江楠幼苗抗寒生理的影响  

Effect of exogenous salicylic acid on the cold resistance physiology of Phoebe chekiangensis

作　　者：顾雯 高捍东[1,2] 薛晓明 GU Wen;GAO Handong;XUE Xiaoming(Co-Innovation Center for Sustainable Forestry in Southern China,Nanjing Forestry University,Nanjing,Jiangsu 210037,China;College of Forestry,Nanjing Forestry University,Nanjing,Jiangsu 210037,China;College of Criminal Science and Technology,Nanjing Police College,Nanjing,Jiangsu 210023,China)

机构地区：[1]南京林业大学南方现代林业协同创新中心,江苏南京210037 [2]南京林业大学林学院,江苏南京210037 [3]南京警察学院刑事科学技术学院,江苏南京210023

出　　处：《森林与环境学报》2024年第1期79-84,共6页Journal of Forest and Environment

基　　金：江苏省林业科技创新与推广项目“楠属珍贵用材树种选育及高效栽培”(LYKJ〔2019〕46)。

摘　　要：为探究低温条件下外源水杨酸(salicylic acid,SA)对浙江楠(Phoebe chekiangensis)幼苗抗寒生理的影响,以期为浙江楠的保护、引种、栽培和开发利用提供科学依据。设置5个SA浓度(0、0.1、0.5、1.0、2.0 mmol·L^(-1))对浙江楠1年生容器苗进行连续3 d的叶面喷施处理,随后利用人工气候室模拟0℃低温,再进行连续7 d的低温胁迫处理,以20℃常温为对照(CK),测定相对电导率(REC)、叶绿素(Chl)含量、丙二醛(MDA)含量、过氧化物酶(POD)活性、超氧化物歧化酶(SOD)活性、可溶性蛋白(SP)含量、可溶性糖(SS)含量、脯氨酸(Pro)含量等生理指标,对浙江楠相关抗寒生理指标进行了相关性和主成分分析。结果表明,适宜浓度的外源SA可以有效缓解REC、MDA含量的上升及Chl含量的下降,促进抗氧化酶活性增加及渗透调节物质积累,减轻低温造成的胁迫伤害。主成分分析结果表明,外源SA预处理在0℃胁迫下能有效提高浙江楠幼苗的抗寒能力,其中1 mmol·L^(-1)的SA处理具有最大缓解效应。To investigate the regulatory effect of exogenous salicylic acid(SA)on the cold resistance physiology of Phoebe chekiangensis under low temperature conditions,in order to provide scientific basis for the protection,introduction,cultivation,and development of P.chekiangensis.Five SA concentrations(0,0.1,0.5,1.0,2.0 mmol·L^(-1))were set to spray the annual container seedlings of P.chekiangensis on the leaf surface for three consecutive days.Then,the climatron was used to simulate the low temperature at 0℃,and then the low temperature stress treatment was carried out for seven consecutive days.The relative conductivity(REC),chlorophyll content(Chl),Malondialdehyde(MDA),peroxidase(POD),Superoxide dismutase(SOD),soluble protein(SP),soluble sugar(SS),proline(Pro)and other physiological indicators.Then the correlation and PCA of physiological indexes of cold resistant of P.chekiangensis were carried out.The results indicate that exogenous SA at appropriate concentrations can effectively alleviate the upward trend of REC and MDA content,as well as the downward trend of Chl,increase the activity and accumulation of antioxidant enzyme and osmoregulation substances,and alleviate stress damage caused by low temperature.The results of principal component analysis showed that exogenous SA pretreatment could effectively improve the cold resistance of P.chekiangensis under 0℃stress,with a SA treatment of 1 mmol·L^(-1)having the greatest alleviating effect.

关 键 词：浙江楠 水杨酸 低温胁迫 生理特性 耐寒性 

分 类 号：S792.24[农业科学—林木遗传育种]