甘草颗粒特征图谱及其质量相关性研究  

Study on the characteristic graph and quality correlation of Radix Glycyrrhizae granules

作　　者：雷露 许浩翔 冉江[1] 姜玲玲 艾蓉 罗文菊 章厉劼 余波 LEI Lu;XU Haoxiang;RAN Jiang;JIANG Lingling;AI Rong;LUO Wenju;ZHANG Lijie;YU Bo(Institute of Animal Husbandry and Veterinary Medicine,Guizhou Academy of Agricultural Sciences,Guiyang Guizhou 550000,China;Guizhou Veterinary Medicine and Feed Testing Institute,Guiyang Guizhou 550000,China)

机构地区：[1]贵州省农业科学院畜牧兽医研究所,贵州贵阳550000 [2]贵州省兽药饲料检测所,贵州贵阳550000

出　　处：《中兽医医药杂志》2024年第1期18-22,共5页Journal of Traditional Chinese Veterinary Medicine

基　　金：贵州省科技厅项目(黔科合成果〔2021〕一般039,黔科合成果〔2022〕一般008);贵州省农业农村厅项目“贵州省现代肉牛产业技术体系建设”(GZCYTX-0302)。

摘　　要：建立甘草颗粒中甘草苷、甘草酸铵含量检测方法并进行指纹图谱研究。采用高效液相色谱法,色谱柱为Eclipse Plus C_(18)柱(4.6 mm×250.0 mm,5μm),检测波长237 nm,以乙腈(A)-0.05%磷酸(B)为流动相,梯度洗脱,流速为1.0 mL/min,柱温为30℃,进样量10μL。结果显示,10批样品有12个共有峰,相似度大于0.901。经对照品比对,指认甘草苷和甘草酸铵两个指标性成分并对其进行定量分析,线性关系良好(R^(2)>0.9998),平均加样回收率为99.67%~102.42%。该方法操作简单、快捷,结果准确,可以甘草颗粒中甘草苷、甘草酸铵含量的测定对甘草颗粒进行质量评价。The aim was to establish the determination method and fingerprint of liquiritin and ammonium glycyrrhizinate in Radix Glycyrrhizae granules.Methods High performance liquid chromatography(HPLC)was performed on Eclipse Plus C_(18) column(4.6 mm×250.0 mm,5μm)with detection wavelength of 237 nm.Acetonitrile(A)-0.05%phosphoric acid(B)was used as mobile phase.Gradient elution was adopted,the flow rate was 1.0 mL/min,the column temperature chamber was 30℃,and the sample size was 10μL.The results showed that there were 12 common peaks in 10 batches of samples,and the similarity was greater than 0.901.Two index components,liquiritin and ammonium glycyrrhizinate,were identified and quantitatively analyzed.The linear relationship was good(R^(2)>0.9998),and the average recovery rate was 99.67%-102.42%.The experiment is simple,quick and accurate.It can be used to determine the content of liquiritin and ammonium glycyrrhizinate in Radix Glycyrrhizae granules and evaluate the quality of Radix Glycyrrhizae granules.

关 键 词：甘草颗粒 甘草苷 甘草酸铵 指纹图谱 

分 类 号：S853.7[农业科学—临床兽医学]