RP-HPLC法同时测定茵栀解毒颗粒中绿原酸和栀子苷的含量  

Simultaneous determination of chlorogenic acid and geniposide in Yinzhi Jiedu granules by RP-HPLC

作　　者：赵丽丽 兰新财 叶菁 缪雨臻 赖笑美 章红兵[2] 李思鸿 安丽娜 ZHAO Lili;LAN Xincai;YE Jing;MIAO Yuzhen;LAI Xiaomei;ZHANG Hongbing;LI Sihong;AN Lina(Zhejiang Jindakang Animal Health Co.,Ltd.,Jinhua Zhejiang 321016,China;Jinhua Polytechnic,Jinhua Zhejiang 321007,China;Zhejiang A&F University,Hangzhou Zhejiang 311300,China)

机构地区：[1]浙江金大康动物保健品有限公司,浙江金华321016 [2]金华职业技术学院,浙江金华321007 [3]浙江农林大学,浙江杭州311300

出　　处：《中兽医医药杂志》2024年第1期70-73,共4页Journal of Traditional Chinese Veterinary Medicine

摘　　要：建立RP-HPLC法同时测定茵栀解毒颗粒中绿原酸和栀子苷的含量。采用反相高效液相色谱法,色谱柱为C_(18)柱(250.0 mm×4.6 mm,5μm),柱温30℃,流动相为乙腈‒甲醇‒0.1%磷酸(11∶8∶81),等度洗脱,检测波长为(235±1)nm,流速为1 mL/min。结果显示,绿原酸的线性范围为0.0943~0.7544μg,r=0.999999(n=5),平均回收率为99.25%(n=6);栀子苷线性范围为0.1971~1.5766μg,r=0.999999(n=5),平均回收率为99.56%(n=6)。该方法检测效率高、专属性强、重复性好,为茵栀解毒颗粒的质量评价提供了简便、准确、可靠的定量方法。The objective was to establish a method for simultaneous determination of chlorogenic acid and geniposide in Yinzhi Jiedu granules by RP-HPLC.RP-HPLC method was developed.The separation was performed on a C_(18) column(250.0 mm×4.6 mm,5μm)with isocratic elution using acetomitrile-methanol-0.1%phosphate solution(11∶8∶81)as mobile phase,and column temperature was 30℃.The flow rate was 1 mL/min and the UV absorbance was monitored at(235±1)nm.The results showed that the linear range of chorogenic acid was 0.0943-0.7544μg,r=0.999999(n=5),the mean recovery was 99.25%(n=6);that of geniposide was 0.1971-1.5766μg,r=0.999999(n=5),and the mean recovery was 99.56%(n=6).The method can be used for quality control of Yinzhi Jiedu granules with simplicity,accuracy and reliability.

关 键 词：茵栀解毒颗粒 绿原酸 栀子苷 RP-HPLC 

分 类 号：S853.7[农业科学—临床兽医学]