铁甲秋海棠花粉离体萌发研究  

In vitro Germination of Begonia masoniana Pollen

作　　者：吕燕玲 LÜYan-ling(Xiamen Botanical Garden,Xiamen 361003,Fujian China)

机构地区：[1]厦门市园林植物园,福建厦门361003

出　　处：《亚热带植物科学》2023年第5期408-412,共5页Subtropical Plant Science

基　　金：厦门市社会发展领域指导性项目(3502Z20234ZD4001)。

摘　　要：为探究铁甲秋海棠Begonia masoniana花粉离体萌发适宜条件,采用液体培养法,通过单因素试验考察蔗糖、硼酸、PEG-4000、氯化钙浓度以及不同培养时间对花粉管生长的影响。结果表明,铁甲秋海棠花粉离体萌发的最适培养基为5%蔗糖+0.01%硼酸+5%PEG-4000+0.003%氯化钙;最佳观察时间为120 min。利用该培养基进行培养,铁甲秋海棠花粉萌发率达90.86%,花粉管长度达511.51μm。该研究结果可为同属其他种类秋海棠的花粉研究及育种提供参考。To explore the suitable conditions for in vitro germination of Begonia masoniana pollen,a liquid culture method was used to investigate the effects of sucrose,boric acid,PEG-4000,calcium chloride concentration,and different cultivation times on pollen tube growth through single factor experiments.The results showed that the optimal medium for pollen germination was sucrose 5%,boric acid 0.01%,PEG-40005%,calcium chloride 0.003%.The optimal observation time was 120 min.The pollen germination rate of B.masoniana was 90.86%.The length of the pollen tube was 511.51μm.Our results could provide reference for pollen research and breeding of other begonias.

关 键 词：铁甲秋海棠 花粉萌发 花粉管生长 离体培养 

分 类 号：Q945.6[生物学—植物学]