抵抗素样分子α对小鼠主动脉内皮细胞形成新生血管能力的影响及机制探讨  

Effects and Mechanism of Resistin-like Moleculesαon Endothelial Cell Angiogenesis in Mouse Aorta

作　　者：段淑香 孟海燕 孙萍 张宁 牛洪梅 赵中松 李浩元 马永晖 Duan Shuxiang;Meng Haiyan;Sun Ping;Zhang Ning;Niu Hongmei;Zhao Zhongsong;Li Haoyuan;Ma Yonghui(Department of Cardiopulmonary rehabilitation,Shandong Provincial Third Hospital,Jinan 250031)

机构地区：[1]山东省立第三医院心肺康复科,济南250031

出　　处：《国际老年医学杂志》2024年第1期41-45,共5页International Journal of Geriatrics

基　　金：山东省医药卫生科技发展计划项目(202103010999)。

摘　　要：目的探讨抵抗素样分子α(又称FIZZ1)对小鼠主动脉内皮细胞形成新生血管能力的影响及机制。方法分别使用10 nmol/L、20 nmol/L和40 nmol/L的FIZZ1刺激小鼠主动脉内皮细胞,使用内皮细胞成管实验和内皮细胞迁移实验体外验证FIZZ1在内皮细胞迁移及血管形成中的作用;采用高通量测序检测FIZZ1对小鼠主动脉内皮细胞信号通路的影响,筛选出表达有显著差异的信号通路。结果在不同浓度的FIZZ1刺激下,小鼠主动脉内皮细胞血管形成的面积逐步增加,40 nmol/L的FIZZ1促进血管生成的作用最强。随着FIZZ1浓度的增加,内皮细胞迁移数量增多,迁移能力逐步增强。高通量基因测序检测中发现共有1253个基因出现了差异性表达,包括427个上调基因和826个下调基因,其机制可能与COL1A1、FLT4、ITGA3等基因表达显著性差异有关。结论FIZZ1能在体外使小鼠主动脉内皮细胞形成新生血管,其机制可能与COL1A1、FLT4、ITGA3等基因表达显著性差异有关。Objective To explore the effects and mechanism of resistin-like moleculesα(also known as FIZZ1)on the ability of mouse aortic endothelial cells to form new blood vessels.Methods Mouse was stimulated by FIZZ1 at 10 nmol/L,20 nmol/L and 40 nmol/L.The role of FIZZ1 in endothelial cell migration and blood vessel formation was verified by using endothelial cell tube formation experiments and endothelial cell migration experiments in vitro;The effect of FIZZ1on signaling in mouse aortic endothelial cells was detected by high-throughput sequencing,and pathways with significant differential expression signaling were selected.Results Under different concentrations of FIZZ1,the area of blood vessel formation in mouse aortic endothelial cells gradually increased,and 40 nmol/L FIZZ1/RELMαhad the strongest effect in promoting angiogenesis.With the increasing concentration of FIZZ1,the number of endothelial cells migrated increased,and the migration capacity gradually increased.A total of 1253 genes were found to be differentially expressed,including 427 up-regulated genes and 826 down-regulated genes.The mechanism may be related to the significant expression difference of COL1A1,FLT 4 and ITGA 3.Conclusion FIZZ1 can induce neovascularization of mouse aortic endothelial cells in vitro,and its mechanism may be related to significant differences in gene expression such as COL1A1,FLT4,and ITGA3.

关 键 词：抵抗素样分子α 血管新生 内皮细胞 主动脉 小鼠 

分 类 号：R543[医药卫生—心血管疾病]