黄芪-丹参通过PI3 K/AKT介导对自发性高血压大鼠肾损伤的作用机制研究  

Astragali Radix-Salviae Miltiorrhizae Radix et Rhizoma Protects Rats from Hyper-tensive Nephropathy via PI3K/AKT Signaling Pathway

作　　者：任永昊 韩聪 REN Yong-hao;HAN Cong(Second Department of Nephrology,Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan Shandong 250014)

机构地区：[1]山东中医药大学附属医院肾内二科,山东济南250014

出　　处：《世界中西医结合杂志》2023年第11期2208-2213,2222,共7页World Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金项目资助(82204886)。

摘　　要：目的探讨黄芪-丹参对自发性高血压大鼠肾损伤的作用和机制。方法将72只SPF级自发性高血压大鼠和Wistar-Kyoto(WKY)大鼠随机分为对照组(对照WKY大鼠)、模型组(自发性高血压大鼠)、黄芪-丹参低剂量组(自发性高血压大鼠,1.7 g/kg的黄芪-丹参处理)、黄芪-丹参中剂量组(自发性高血压大鼠,3.4 g/kg的黄芪-丹参处理)、黄芪-丹参高剂量组(自发性高血压大鼠,11.2 g/kg的黄芪-丹参处理)、黄芪-丹参高剂量+740Y-P组(自发性高血压大鼠,11.2 g/kg的黄芪-丹参、740Y-P处理),每组各12只。Western blot检测p-PI3 K、PI3 K、p-AKT、AKT、CollagenⅠ蛋白表达,全自动生化分析仪检测血清中血尿氮素(Hematuria nitro-gen,BUN)、血肌酐值(Blood creatinine value,SCr)含量,免疫比浊法检测尿液尿微量白蛋白(Urine microalbumin,U-mAlb),Masson染色评价肾小球胶原纤维沉积评分,TBA法检测丙二醛(Malondialdehyde,MDA)含量,ELISA方法测定肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α)、白介素-6(Interleukin-6,IL-6)含量;生化法检测氧化应激因子含量。结果黄芪-丹参低剂量组、黄芪-丹参中剂量组、黄芪-丹参高剂量组大鼠BUN、SCr及U-mAlb均低于模型组,黄芪-丹参高剂量+740Y-P组大鼠BUN、SCr及U-mAlb均明显高于黄芪-丹参高剂量组,差异有统计学意义(P<0.05),黄芪-丹参低剂量组、黄芪-丹参中剂量组、黄芪-丹参高剂量组大鼠肾组织中TNF-α、IL-6含量均低于模型组,黄芪-丹参高剂量+740Y-P组大鼠肾组织中TNF-α、IL-6含量均明显高于黄芪-丹参高剂量组,差异有统计学意义(P<0.05)。模型组大鼠肾组织中CollagenⅠ、CollagenⅣ、TGF-β1蛋白表达量和肾小球胶原纤维沉积评分、肾小管间质病变评分均明显高于对照组,黄芪-丹参低剂量组、黄芪-丹参中剂量组、黄芪-丹参高剂量组大鼠肾组织中CollagenⅠ、CollagenⅣ、TGF-β1蛋白表达量和肾小球胶原纤维沉积评分、肾小管间质病Objective To investigate the effect and mechanism of Astragali Radix and SALviamiltiorrhiza on renal injury in spontaneously hypertensive rats.Method A total of 72 SPF-grade spontaneously hypertensive rats and Wistar-Kyoto(WYK)rats were assigned into a control group(WKY rats),a model group(spontaneous hypertensive rats),low-(1.7 g/kg),medium-(3.4 g/kg),and high-dose(11.2 g/kg)Astragali Radix-Salviae Miltiorrhizae Radix et Rhizoma groups(spontaneous hypertensive rats),and a high-dose(11.2 g/kg)Astragali Radix-Salviae Miltiorrhizae Radix et Rhizoma+740Y-P group(spontaneous hypertensive rats),with 12 rats in each group.Western blotting was employed to determine the protein levels of phosphorylated phosphatidylinositol 3-kinase(p-PI3 K),PI3 K,phosphorylated protein kinase B(p-AKT),AKT,and collagen I.A fully automatic biochemical analyzer was used to measure the levels of blood urea nitrogen(BUN)and serum creatine(Scr).Urine microalbumin(U-mAlb)was determined by immunoturbidimetry.Masson staining was performed to score the glomerular collagen fiber deposition.The content of malondialdehyde(MDA)was determined by the TBA method.Enzyme-linked immunosorbent assay was employed to measure the levels of tumor necrosis factor-alpha(TNF-α)and interleukin-6(IL-6).Biochemical Method were employed to measure the content of oxidative stress factors.Results Compared with the model group,low-,medium-,and high-dose Astragali Radix-Salviae Miltiorrhizae Radix et Rhizoma lowered the levels of BUN,SCr,and U-mAlb.The high-dose Astragali Radix-Salviae Miltiorrhizae Radix et Rhizoma group had lower levels of BUN,SCr,and U-mAlb than the high-dose Astragali Radix-Salviae Miltiorrhizae Radix et Rhizoma+740Y-P group(P<0.05).The levels of TNF-αand IL-6 in renal tissue of rats in the low-,medium-,and high-dose Astragali Radix-Salviae Miltiorrhizae Radix et Rhizoma groups were lower than those in model group,and the TNF-αand IL-6 levels in the high-dose Astragali Radix-Salviae Miltiorrhizae Radix et Rhizoma+740Y-P group were higher than those

关 键 词：黄芪-丹参 PI3 K/AKT信号 自发性高血压 肾损伤 动物模型 

分 类 号：R285.5[医药卫生—中药学]