重金属混合暴露与育龄男性精浆果糖的关联  

Relationship between mixed exposure to heavy metals and seminal fructose in men of childbearing age

作　　者：曹金虎 林风龙 岳忠义 张平洋 韩玉芬 张光辉 任静朝 CAO Jinhu;LIN Fenglong;YUE Zhongyi;ZHANG Pingyang;HAN Yufen;ZHANG Guanghui;REN Jingchao(School of Public Health,Xinxiang Medical University,Xinxiang,Henan 453003,China;Puyang Maternal and Child Health Hospital Reproductive Center,Puyang,Henan 457000,China;Department of Military Preventive Medicine,Army Medical University,Chongqing 400038,China;School of Public Health,Chongqing Medical University,Chongqing 400016,China)

机构地区：[1]新乡医学院公共卫生学院,河南新乡453003 [2]濮阳市妇幼保健院生殖中心,河南濮阳457000 [3]陆军军医大学军事预防医学系,重庆400038 [4]重庆医科大学公共卫生学院,重庆400016

出　　处：《环境与职业医学》2023年第11期1270-1277,共8页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金面上项目(82273663);国家自然科学基金青年项目(82003489)。

摘　　要：[背景]人体通常同时暴露于多种重金属环境之中,不同种类和浓度的重金属元素在人体吸收和代谢过程中可能产生复杂的交互作用。研究表明金属暴露可能导致精液质量损伤,精浆果糖是精子运动的重要能量来源,分析重金属混合暴露与精浆果糖的关联,对探索金属暴露致精液质量损伤的机制具有一定的意义。[目的]了解河南省濮阳市育龄男性常见重金属暴露现状,探索分析重金属混合暴露及其交互作用与育龄男性精浆果糖水平的相关性。从濮阳市妇幼保健院生殖中心招募志愿者进行横断面研究,利用问卷调查研究对象的一般人口学特征、吸烟、饮酒等资料。采集精液样本,检测精浆中钒(V)、锰(Mn)、钴(Co)、镍(Ni)、锌(Zn)、硒(Se)、银(Ag)、镉(Cd)、钡(Ba)、铊(Tl)、铁(Fe)、铅(Pb)12种金属浓度与精浆果糖水平。在校正混杂因素后使用贝叶斯核机器回归(BKMR)模型评估精浆重金属混合暴露及其交互作用对精浆果糖的影响。共825名成年男性纳入本次研究,V、Mn、Co、Ni、Zn、Se、Ag、Cd、Ba、Tl、Fe、Pb共12种金属精浆暴露浓度M(P_(25),P_(75))分别为0.39(0.28,0.54)、12.31(8.92,17.52)、0.26(0.18,0.38)、5.15(3.32,8.64)、182159.80(121847.80,199144.50)、13.61(10.55,17.68)、0.03(0.02,0.04)、0.34(0.27,0.46)、8.64(5.94,13.43)、0.06(0.05,0.08)、168.74(114.17,259.45)、1.69(1.15,2.36)μg·L^(-1)。Spearman相关分析结果提示,精浆V、Mn、Co、Zn、Se、Ba、Tl、Fe与精浆果糖之间呈现负相关关系(P<0.05),r(95%CI)分别为-0.044(-0.087~-0.001)、-0.129(-0.171~-0.087)、-0.055(-0.099~-0.012)、-0.099(-0.143~-0.056)、-0.053(-0.097~-0.010)、-0.068(-0.111~-0.025)、-0.095(-0.138~-0.052)、-0.082(-0.125~-0.039)。多重线性回归结果提示,精浆Cd、Mn、Zn、Ag、Ba、Tl、Fe暴露与精浆果糖之间呈现负相关关系(P<0.05),β(95%CI)分别为-0.551(-0.956~-0.147)、-0.315(-0.419~-0.212)、-0.187(-0.272~-0.103)、-0.161(-0.301~-0.021)�[Background]The human body is usually exposed to a variety of heavy metals at the same time,and different types and concentrations of heavy metals may have complex interactions during their absorption and metabolism in the human body.Seminal fructose is an important energy source for sperm movement.A large number of studies have shown that metal exposure may impair semen quality,and seminal fructose is an important factor affecting male reproduction,so it is necessary to investigate the relationship between mixed heavy metal exposure and seminal fructose to explore the mechanism of semen quality damage caused by metal exposure.[Objective]To understand the status of common heavy metal exposure in men of childbearing age in Puyang City,Henan Province,and to study the relationship between mixed exposure to heavy metals and seminal fructose,as well as potential interactions among heavy metals.[Methods]Volunteers were recruited from the Puyang Maternal and Child Health Hospital Reproductive Center for a cross-sectional survey on general demographic characteristics,smoking,alcohol consumption,and other information.Semen samples were collected to detect 12 metals such as vanadium(V),manganese(Mn),cobalt(Co),nickel(Ni),zinc(Zn),selenium(Se),silver(Ag),cadmium(Cd),barium(Ba),thallium(Tl),iron(Fe),and lead(Pb)in seminal plasma and seminal fructose.After correcting for selected confounding factors,a Bayesian kernel machine regression(BKMR)model was used to evaluate the impact of seminal plasma heavy metal mixed exposure and its interactions on seminal fructose.[Results]A total of 825 adult males were enrolled.The concentrations in M(P_(25),P_(75))of V,Mn,Co,Ni,Zn,Se,Ag,Cd,Ba,Tl,Fe,and Pb in seminal plasma were 0.39(0.28,0.54),12.31(8.92,17.52),0.26(0.18,0.38),5.15(3.32,8.64),182159.80(121847.80,199144.50),13.61(10.55,17.68),0.03(0.02,0.04),0.34(0.27,0.46),8.64(5.94,13.43),0.06(0.05,0.08),168.74(114.17,259.45),and 1.69(1.15,2.36)μg·L^(-1) respectively.The Spearman correlation results indicated that there was a negative cor

关 键 词：重金属 混合暴露 精浆果糖 交互作用 贝叶斯核机器回归 

分 类 号：R12[医药卫生—环境卫生学]