SUPER WOMAN 2(SPW2)maintains organ identity in spikelets by inhibiting the expression of floral homeotic genes OsMADS3,OsMADS58,OsMADS13,and DROOPING LEAF  

作　　者：Hui Zhuang Jinsong Lan Qiuni Yang Xiaoyu Zhao Yuhuan Li Jingya Zhi Yalin Shen GuanghuaHe Yunfeng Li 

机构地区：[1]Rice Research Institute,Key Laboratory of Application and Safety Control of Genetically Modified Crops,Academy of Agricultural Sciences,Southwest University,Chongqing 400715,China

出　　处：《Journal of Integrative Agriculture》2024年第1期59-76,共18页农业科学学报（英文版）

基　　金：supported by the Chongqing Modern Agricultural Industry Technology System,China(CQMAITS202301);the National Natural Science Foundation of China(32100287 and 31971919);the Natural Science Foundation of Chongqing,China(cstc2020jcyj-jq X0020 and cstc2021ycjh-bgzxm0066);the China Postdoctoral Science Foundation Funded Project(2020M683219);the Fundamental Research Funds for the Central Universities,China(SWU-XDJH202315)。

摘　　要：Flower organ identity in rice is mainly determined by the A-,B-,C-and E-class genes,with the majority encoding MADS-box transcription factors.However,few studies have investigated how the expression of these floral organ identity genes is regulated during flower development.In this study,we identified a gene named SUPER WOMAN 2(SPW2),which is necessary for spikelet/floret development in rice by participating in the regulation of the expression of pistil identity genes such as OsMADS3,OsMADS13,OsMADS58 and DL.In the spw2 mutant,ectopic stigma/ovary-like tissues were observed in the non-pistil organs,including sterile lemma,lemma,palea,lodicule,and stamen,suggesting that the identities of these organs were severely affected by mutations in SPW2.SPW2 was shown to encode a plant-specific EMF1-like protein that is involved in H3K27me3 modification as an important component of the PRC2 complex.Expression analysis showed that the SPW2 mutation led to the ectopic expression of OsMADS3,OsMADS13,OsMADS58,and DL in non-pistil organs of the spikelet.The ChIP-qPCR results showed significant reductions in the levels of H3K27me3 modification on the chromatin of these genes.Thus,we demonstrated that SPW2 can mediate the process of H3K27me3 modification of pistil-related genes to regulate their expression in non-pistil organs of spikelets in rice.The results of this study expand our understanding of the molecular mechanism by which SPW2 regulates floral organ identity genes through epigenetic regulation.

关 键 词：rice(Oryza sativa) SPIKELET organ identity H3K27me3 

分 类 号：S511[农业科学—作物学]