胎盘绒毛跨膜蛋白CD81、Ang与复发性流产女性早孕期复发流产的关系  

Relationship between placental villi transmembrane protein CD81,Ang levels and recurrent abortion in early pregnancy of women with recurrent spontaneous abortion

作　　者：张晓英 董小敏 赵盼盼 于芳 ZHANG Xiao-ying;DONG Xiao-min;ZHAO Pan-pan;YU Fang(Department of Obstetrics,Xianyang Central Hospital,Xianyang 712000,Shaanxi,China;Department of Laboratory Medicine,Xianyang Central Hospital,Xianyang 712000,Shaanxi,China)

机构地区：[1]咸阳市中心医院,产科,陕西咸阳712000 [2]咸阳市中心医院,检验科,陕西咸阳712000

出　　处：《川北医学院学报》2024年第1期38-41,共4页Journal of North Sichuan Medical College

基　　金：陕西省科学技术发展计划项目(2008K15-02)。

摘　　要：目的:探究胎盘绒毛跨膜蛋白CD81、血管生成素(Ang)与复发性流产(RSA)女性早孕期复发流产的关系。方法:选择126例RSA患者为研究对象(RSA组),另选取94例正常早孕行人工流产术者为对照组。采用RT-qPCR、Western blot法检测胎盘绒毛组织CD81、Ang-1、Ang-2 mRNA及蛋白表达水平,采用电化学发光法检测血清人绒毛膜促性腺激素(HCG)水平,计算Ang-1/Ang-2 mRNA及蛋白比值,比较两组患者检测结果。结果:RSA组CD81、Ang-1、Ang-2的mRNA及蛋白表达和Ang-1/Ang-2 mRNA及蛋白表达比值均高于对照组,血清HCG水平低于对照组(P<0.05)。RSA组CD81 mRNA与Ang-1、Ang-2 mRNA呈正相关(P<0.05);RSA组CD81蛋白表达量与Ang-1、Ang-2蛋白表达量呈正相关(P<0.05),血清HCG水平与其他指标无相关性(P>0.05)。CD81、Ang-1/Ang-2的mRNA及蛋白和血清HCG均可用于预测RSA女性早孕期复发流产。结论:RSA女性早孕期复发流产与胎盘绒毛CD81、Ang-1、Ang-2 mRNA及蛋白表达量异常高表达有关,CD81、Ang-1、Ang-2可能影响胎盘血管形成而致流产。Objective:To investigate the relationship between the levels of placental villi transmembrane protein CD81,angiopoietin(Ang)and recurrent abortion in early pregnancy of women with recurrent spontaneous abortion(RSA).Methods:126 patients with RSA(RSA group)and 94 subjects with normal early pregnancy(control group)were selected.The mRNA and protein expression levels of CD81,Ang-1 and Ang-2 in placental villi tissues were detected by real-time fluorescence quantitative polymerase chain reaction and Western blot,and levels of serum human chorionic gonadotropin(HCG)was detected by electrochemiluminescence method.The mRNA and protein expression ratios of Ang-1/Ang-2 were calculated,and the detection results were compared.Results:The mRNA and protein expression levels of CD81,Ang-1 and Ang-2 in placental villi,Ang-1/Ang-2 mRNA ratio and Ang-1/Ang-2 protein expression ratio in RSA group were higher than those in control group,while the level of serum HCG was lower than that in control group(P<0.05).The CD81 mRNA in RSA group was positively correlated with the mRNA levels of Ang-1 and Ang-2(P<0.05).The protein expression of CD81 in RSA group was positively correlated with the protein expressions of Ang-1 and Ang-2(P<0.05),and serum HCG level was not correlated with the other indicators(P>0.05).The mRNA and protein expression levels of CD81 and Ang-1/Ang-2 and serum HCG could be used to predict recurrent abortion in women with RSA during early pregnancy.Conclusion:Recurrent abortion in early pregnancy of women with RSA is related to abnormal high mRNA and protein expressions of CD81,Ang-1 and Ang-2 in placental villi.CD81,Ang-1 and Ang-2 may affect placental angiogenesis and then lead to abortion.

关 键 词：复发性流产 早孕期 胎盘绒毛 跨膜蛋白CD81 血管生成素 人绒毛膜促性腺激素 

分 类 号：R714.21[医药卫生—妇产科学]