失笑散化学成分分析  

Analysis of Chemical Constituents in Shixiao Powder

作　　者：王清[1] 魏星 陈佩东[2] 曹雨诞[2] WANG Qing;WEI Xing;CHEN Peidong;CAO Yudan(Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing,Jiangsu,China 210029;School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing,Jiangsu,China 210023)

机构地区：[1]南京中医药大学附属医院,江苏南京210029 [2]南京中医药大学药学院,江苏南京210023

出　　处：《中国药业》2024年第2期55-60,共6页China Pharmaceuticals

基　　金：国家自然科学基金[81873001]。

摘　　要：目的分析失笑散的化学成分。方法采用超高效液相色谱串联三重四极杆飞行时间质谱(UPLC-Q-TOF-MS)法,色谱柱为Cortecs UPLC T3柱(100 mm×2.1 mm,1.6μm),流动相为0.1%甲酸水溶液-乙腈(梯度洗脱),流速为0.3 mL/min,柱温为30℃,进样量为2μL;电喷雾离子源(ESI),正负离子模式。结果共解析出53个化学成分,其中组方药材蒲黄中23个,五灵脂中14个,其余16个为两者共有。蒲黄独有成分及其与五灵脂共有成分均以黄酮类成分为主,而五灵脂独有成分以萜类成分为主。结论该方法操作简便,重复性好,可为进一步研究失笑散的效应-物质基础提供依据。Objective To analyze the chemical constituents of Shixiao Powder.Methods Ultra-high-performance liquid chromatography-triple quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS)was adopted.The chromatographic column was Cortecs UPLC T3 column(100 mm×2.1 mm,1.6μm),the mobile phase was 0.1%formic acid aqueous solution-acetonitrile(gradient elution),the flow rate was 0.3 mL/min,the column temperature was 30℃,the injection volume was 2μL,and the electro-spray ionization(ESI)was adopted with the positive and negative ion modes.Results A total of 53 chemical constituents were obtained,including 23 in Typhae Pollen,14 in Faeces Trogopteror,and 16 were common in the two Chinese medicinal materials.The characteristic constituents of Typhae Pollen and the common constituents of the two Chinese medicinal materials were mainly flavonoids,while the characteristic constituents of Faeces Trogopteror were mainly terpenoids.Conclusion This method is easy and repeatable,which can provide a basis for the further research of the effect-material basis of Shixiao Powder.

关 键 词：失笑散 蒲黄 五灵脂 化学成分 超高效液相色谱串联三重四极杆飞行时间质谱法 

分 类 号：R917[医药卫生—药物分析学]