新疆布尔根河狸国家级自然保护区狭耳鼠耳蝠线粒体基因组研究  

Study on the Mitochondrial Genomes of Myotis blythii in the Buergen Beaver National Nature Reserve,Xinjiang

作　　者：阿斯姑丽·阿布都热合曼 初雯雯 戚英杰 初红军[1,2] 吴红潘 何承斌 ABDURAHMAN Asimgul;CHU Wenwen;QI Yingjie;CHU Hongjun;WU Hongpan;HE Chengbin(College of Life Science and Technology,Xinjiang University,Urumqi,830046,China;Xinjiang Academy of Forestry,Urumqi,830002,China;Grassland Station of Xinjiang Uygur Autonomous Region,Urumqi,830049,China;College of Ecology and Nature Conservation,Beijing Forestry University,Beijing,100083,China;Nature Conservation Association of Altay Region,Altay,836500,China;Rural Revitalization Development Center,Agriculture and Rural Bureau,Shantou,515071,China;Management Bureau of Buergen Beaver National Nature Reserve,Altay,836500,China)

机构地区：[1]新疆大学生命科学与技术学院,乌鲁木齐830046 [2]新疆林科院森林生态研究所,乌鲁木齐830002 [3]新疆维吾尔自治区草原总站,乌鲁木齐830049 [4]北京林业大学生态与自然保护学院,北京100083 [5]新疆阿尔泰地区自然保护协会,阿勒泰836500 [6]汕头市濠江区农业农村局乡村振兴发展中心,汕头515071 [7]新疆布尔根河狸国家级自然保护区管理局,阿勒泰836500

出　　处：《野生动物学报》2024年第1期43-49,共7页CHINESE JOURNAL OF WILDLIFE

基　　金：科技部第三次新疆综合科学考察专项项目(2021xjkk1200,2021xjkk1201);国家林业和草原局科学技术司林草科技创新发展研究项目(2022132017);新疆维吾尔自治区国际科技合作项目(20136026-2)。

摘　　要：为提供鼠耳蝠属(Myotis)蝙蝠类遗传及进化分子水平的证据,通过高通量测序法对新疆布尔根河狸国家级自然保护区栖息的狭耳鼠耳蝠(M.blythii)线粒体基因组全序列进行测定和分析。结果显示:狭耳鼠耳蝠线粒体基因组总长为16752 bp,总碱基组成为33.8%A、30.4%T、22.7%C和13.1%G;线粒体基因组由13个蛋白质编码基因、2个r RNA(12S和16S)基因、22个t RNA基因和1个非编码控制区组成;控制区基因长度为948 bp,比大鼠耳蝠(M.myotis)等姊妹物种短很多;与从NCBI下载的其他鼠耳蝠属蝙蝠线粒体基因组序列的系统发育分析比较,狭耳鼠耳蝠与大鼠耳蝠亲缘关系最近。研究结果丰富了鼠耳蝠属线粒体基因组信息,明确了新疆狭耳鼠耳蝠在鼠耳蝠属内的系统进化地位和亲缘关系,为蝙蝠科(Vespertilionidae)系统分子进化和后续研究提供基础数据。To provide molecular level evidence for the genetics and evolution of bats belonging to the genus Myotis,this study used high-throughput sequencing to determine and analyze the mitochondrial genome sequence of M.blythii,a species inhabiting at the Buergen Beaver National Nature Reserve of Xinjiang,China.Results showed that the total length of the mitochondrial genome of M.blythii was 16,752 bp,and the total base groups were 33.8% A,30.4% T,22.7% C,and 13.1% G.The mitochondrial genome consisted of 13 protein coding genes,2 rRNAs(12S and 16S RNA) genes,22 tRNA genes,and a noncoding control region.The length of the control region gene was 948 bp,which was much shorter than that of sister species such as M.myotis.Based upon mitochondrial genome sequences collected from NCBI for phylogenetic analysis of other bat species belonging to the genus Myotis,the analysis showed that M.blythii and M.myotis had the closest genetic relationship.The research findings of this study have enriched the mitochondrial genome information of this species,clarified the phylogenetic status and phylogenetic relationship of M.blythii in Xinjiang within the genus Myotis,and provided a data basis for the systematic and molecular evolution of Vespertilionidae and subsequent research.

关 键 词：线粒体基因组 狭耳鼠耳蝠 濒危物种 高通量测序 系统发育 

分 类 号：Q953[生物学—动物学]