东莞市HIV-1流行株pol基因序列特征分析  

Sequence analysis of pol gene of HIV-1 strain in Dongguan

作　　者：何松美[1] 梁金虎 钟庆杨 曹焕焕[1] 尹菊侦 王福祥 He Songmei;Liang Jinhu;Zhong Qingyang;Cao Huanhuan;Yin Juzhen;Wang Fuxiang(Department of Infection,Dongguan People's Hospital,Guangdong Dongguan 523058,China;Department of Infectious Diseases,The Third People's Hospital of Shenzhen,The Second Affiliated Hospital of Southern University of Science and Technology,Guangdong Shenzhen 518112,China;Department of Infection,Dongguan Ninth People's Hospital,Guangdong Dongguan 523018,China)

机构地区：[1]东莞市人民医院感染科,广东东莞523058 [2]深圳市第三人民医院(南方科技大学附属第二医院)感染中心,广东深圳518112 [3]东莞市第九人民医院感染科,广东东莞523018

出　　处：《新发传染病电子杂志》2023年第6期9-13,共5页Electronic Journal of Emerging Infectious Diseases

基　　金：国家自然科学基金面上项目(81971915,82272318);深圳市科技研发基金(JCYJ20200109144203815);广东省高水平临床重点专科(深圳市配套建设经费)资助(SZGSPOI1);基于干细胞技术的重大传染病临床救治方案研究(2022YFC2304403,2022YFC2304404)。

摘　　要：目的了解东莞市HIV-1感染者的病毒流行特征及亚型的分布情况。方法收集2019年1月至2021年1月东莞市未接受抗病毒治疗的HIV-1感染者284例,收取其基本人口学信息,采集血液样本并提取病毒RNA,采用巢式聚合酶链反应扩增HIV-1 pol段基因,应用LaserGene、HIV BLAST、MEGA 6.0等生物学软件进行基因序列分析。结果本研究纳入284例样本,最终成功获得269条HIV-1 pol基因序列。269份样本中,86.2%为男性,61.3%年龄<40岁,52.4%为未婚,异性性传播占比49.4%,同性性传播占比47.2%。269条pol基因序列中,共发现8种基因亚型,其中主要亚型为CRF07_BC(48.7%)、CRF01_AE(21.9%)和CRF55_01B(14.1%)。各个亚型感染者在性别和婚姻状况的构成上差异有统计学意义(P<0.05),在年龄和感染途径的构成上差异无统计学意义。结论东莞市HIV-1亚型分布复杂多样,以CRF07_BC、CRF01_AE和CRF55_01B为主,且在不同性别、不同婚姻状况的HIV感染者中存在显著差异。Objective To investigate the epidemiological characteristics of human immunodeficiency virus type 1(HIV-1)and the subtypes distribution among HIV-1-infected individuals in Dongguan.Method From January 2019 to January 2021,284 HIV-1 infected patients who had received antiviral treatment in Dongguan were selected,and was collected their epidemiological information.The viral RNA of HIV-1 was extracted from plasma.The pol gene was amplified by nested polymerase chain reaction.and gene sequence analysis was performed using biological software such as LaserGene,HIV BLAST,and MEGA 6.0.Result A total of 284 samples were collected in this study and finally successfully obtained 269 HIV-1 pol gene sequences.Among the 269 samples,86.2%(232/269)were male,61.3%(165/269)were younger than 40 years old,52.4%(141/269)were unmarried,and 49.4%(133/269)were heterosexual and 47.2%(127/269)were homosexual.Of the 269 pol gene sequences,eight genetic subtypes were identified:131(48.7%)CRF07_BC,59(21.9%)CRF01_AE,38(14.1%)CRF55_01B,19(7.1%)subtype B,12(4.5%)CRF08_BC,and 10(3.7%)other subtypes.Among 133 patients with heterosexual transmission,the predominant subtypes were 59(44.4%)CRF07_BC,32(24.1%)CRF01_AE,and 15(11.3%)CRF55_01B,and among 127 patients with homosexual transmission,the predominant subtypes were 65(51.2%)CRF07_BC,26(20.5%)CRF01_AE,and 23(18.1%)CRF55_01B.Differences in gender and marital status composition among individuals infected with different subtypes were statistically significant(P<0.05),while no statistically significant differences were observed in age and mode of infection composition.Conclusion The distribution of HIV-1 subtypes in Dongguan is complex and diverse,with CRF07_BC,CRF01_AE,and CRF55_01B being predominant.Furthermore,significant differences exist among HIV-infected individuals of different genders and marital statuses.

关 键 词：人类免疫缺陷病毒Ⅰ型 序列分析 亚型分布 

分 类 号：R512.91[医药卫生—内科学]