生物光学成像技术在组织穿透性方面的研究进展  

Advances in Tissue Penetration by Optical Imaging Techniques

作　　者：张玉敏 王富 林俐 叶坚 ZHANG Yu-min;WANG Fu;LIN Li;YE Jian(School of Biomedical Engineering,Shanghai Jiao Tong University,Shanghai 200030,China)

机构地区：[1]上海交通大学生物医学工程学院,上海200030

出　　处：《分析测试学报》2024年第1期19-31,共13页Journal of Instrumental Analysis

基　　金：国家自然科学基金项目(82272054)。

摘　　要：光学成像因灵敏度高、特异性强、无电离辐射、低成本、丰富的候选探针、可获取细胞/分子水平信息和可实时检测等优势,在临床前的基础研究和临床诊断与治疗领域具有巨大的应用价值。但由于生物组织对光子的高散射与高吸收特性,光学成像的组织穿透深度通常非常有限,很大程度上限制了其在深部病变活体生物医学检测方面的应用,研究者们对此做了大量的努力。随着科学技术的发展,光学技术的组织检测深度已覆盖微米到厘米甚至分米以上的范围,在生物检测、成像、诊断、术中导航等领域展现出了广阔的应用前景。该文从常见的光学成像技术入手,对荧光成像、生物/化学发光成像、光声成像以及拉曼成像在组织穿透性方面的研究进展进行了总结与讨论,并对这些光学成像技术未来在组织穿透方面的主要研究方向进行了展望。Optical imaging has great potential for application in the field of preclinical basic research and clinical diagnostics and therapeutics,due to its advantages of high sensitivity and specificity,non-ionizing radiation,simplicity of equipment,low cost,rich nanoprobe candidates,ability to ob-tain cellular/molecular level information and real-time acquisition capability.However,due to the nature of high scattering and absorption of photons in biological tissues,optical imaging is usually limited by the shallow tissue penetration depth,which largely limits its usage for in vivo biomedical detection of deep-seated lesions.A lot of efforts have been done by researchers to overcome this is-sue.This paper summarizes and discusses the progress of various common optical imaging tech-niques,such as fluorescence imaging,bioluminescence/chemiluminescence imaging,photoacous-tic imaging,and Raman imaging,in terms of their research progress in tissue penetration.With the development of science and technology,the tissue detection depths of optical modalities have cov-ered a range from microns to centimeters or even to decimeters,and have shown broad application prospects in the fields of biological detection,imaging,diagnosis,intraoperative navigation,and so on.Finally,the main directions of future research of these optical imaging techniques in tissue.

关 键 词：生物光学成像 组织穿透性 深穿透拉曼光谱 

分 类 号：O657.3[理学—分析化学] R318[理学—化学]