原位合成Au/泡沫镍磁性基底用于表面增强拉曼快速检测血清中免疫抑制剂代谢物  

In Situ Synthesis of Au/Foam Nickel Magnetic Substrate for Rapid Detection of Immunosuppressive Drug Metabolites in Serum by Us⁃ing Surface Enhanced Raman Scattering Spectroscopy

作　　者：刘尧尧 李朵朵 郭小玉 文颖 杨海峰 LIU Yao-yao;LI Duo-duo;GUO Xiao-yu;WEN Ying;YANG Hai-feng(College of Chemistry and Materials Science,Shanghai Normal University,Shanghai 200234,China)

机构地区：[1]上海师范大学化学与材料科学学院,上海200234

出　　处：《分析测试学报》2024年第1期200-206,共7页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(21475088)。

摘　　要：该文采用处理后的泡沫镍为反应模板,直接还原氯金酸溶液,经实验条件优化,原位制备了纳米金-泡沫镍多孔磁性复合表面增强拉曼散射(SERS)基底(Au-NFs)。泡沫镍三维网状具有大的比表面积和粗糙度,可稳定负载纳米金,通过Au—S键化学吸附更多的目标分子6-巯基嘌呤。复合基底的磁特性易于磁分离富集,避免血液共存物对SERS检测6-巯基嘌呤的干扰。利用磁场诱导纳米金-泡沫镍聚集,可进一步优化纳米金局域等离子场分布,形成SERS热点,大大提升了分析灵敏度,对于探针分子4-巯基吡啶的检出限可达5×10^(-9)mol/L。在外磁场优化条件下,基于Au-NFs复合SERS基底定量检测6-巯基嘌呤,在5×10^(-7)~1×10^(-8) mol/L浓度范围内有良好的线性,检出限为1×10^(-9) mol/L。该Au-NFs基底无需样品预处理,可实现血清中6-巯基嘌呤的快速分析,加标回收率在92.8%~107%范围内,表明方法可靠。In this paper,porous magnetic gold nano-foam nickel composite is in situ prepared as sur-face enhanced Raman scattering(SERS)substrate(Au-NFs)by directly reducing chloric acid gold so-lution with treated foam nickel as a reductive reaction template.The three-dimensional network of foam nickel with a large specific surface area and roughness could tightly load gold nanoparticles and chemically adsorb more target molecules 6-mercaptopurine via the Au—S bond.The magnetic proper-ties of the composite substrate make it easy for magnetic separation and enrichment to avoid the inter-ference of blood coexistence species on SERS detection of 6-mercaptopurine.By using magnetic field,porous magnetic Au-NFs can be further optimized the distribution of the local plasma field of gold nanoparticles to form SERS hot spots,which greatly improve the detection sensitivity.For probe mol-ecule 4-mercaptopyridine,the Au-NFs-based SERS detection limit at 5×10-9 mol/L could be reached.Under external magnetic field,with Au-NFs composite substrate,a linear detection of 6-mercaptopurine concentration locates in the range of 5×10^(-7) and 1×10^(-8 )mol/L,with a detection limit of 1×10^(-9) mol/L.Without sample pretreatment,this Au-NFs substrate could be used for rapid analy-sis of 6-mercaptopurine spiked in serum with recoveries ranging from 92.8%to 107%,indicating the reliability of the method.

关 键 词：泡沫镍 金纳米 磁复合材料 6-巯基嘌呤 SERS检测 血清 免疫抑制剂 

分 类 号：O657.37[理学—分析化学] R318[理学—化学]