基于内标型花瓣状间隙增强拉曼粒子的pH传感  

pH Sensing Based on Internal Standard Petal-like Gap-enhanced Raman Particles

作　　者：张铭中 关鹏程 林嘉盛 许珊珊 张凡利 张月皎 李志勇[4] 李剑锋 ZHANG Ming-zhong;GUAN Peng-cheng;LIN Jia-sheng;XU Shan-shan;ZHANG Fan-i;ZHANG Yue-jiao;LI Zhi-yong;LI Jian-feng(College of Optical and Electronic Technology,China Jiliang University,Hangzhou 310018,China;College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China;College of Materials,Xiamen University,Xiamen 361005,China;The First Affiliated Hospital,Xiamen University,Xiamen 361005,China;College of Energy,Xiamen University,Xiamen361005,China;Tan Kah Kee Innovation Laboratory,Xiamen 361005,China)

机构地区：[1]中国计量大学光学与电子科技学院,浙江杭州310018 [2]厦门大学化学化工学院,福建厦门361005 [3]厦门大学材料学院,福建厦门361005 [4]厦门大学第一附属医院,福建厦门361005 [5]厦门大学能源学院,福建厦门361005 [6]嘉庚创新实验室,福建厦门361005

出　　处：《分析测试学报》2024年第1期207-212,共6页Journal of Instrumental Analysis

基　　金：厦门市科技计划项目(35022022YJ03);中央高校基本科研业务费专项资金(20720220139,20720220117)。

摘　　要：汗液是一种可无创测量的分析物,其pH值可作为诊断和监测各种生理状况的重要指标。表面增强拉曼散射技术(SERS)是一种快速、灵敏、无创、无标记的分析检测技术,但存在因信号波动导致的稳定性差等问题。该文通过制备一种内标型花瓣状间隙增强拉曼标签(PGERT)纳米颗粒并构建pH传感器,实现了对人体汗液中pH值的精确实时分析。该传感器通过在间隙结构内部修饰4-巯基苯甲氰(4-MBN)分子获得内标拉曼信号,并基于外部花瓣状壳层表面修饰pH传感分子4-巯基苯甲酸(4-MBA)获得增强的探针信号,显著提高了检测稳定性和灵敏度。该研究先使用原位分析池建立了pH 5.0~8.0条件下的标准分析曲线,相关系数(r2)高达0.99。该传感器对4种不同pH值汗液(pH 5.0~7.0)的检测结果与标准pH计测试结果的误差率分别为(2.17±5.0)%、(4.62±1.1)%、(5.80±0.3)%、(3.68±1.0)%。结果表明,基于该内标型花瓣状间隙纳米拉曼标签的传感器可用于人体汗液的pH值监测,并具有高灵敏度、高稳定性以及分析精确的优点。This study describes a non-invasive,real-time analysis of pH levels in human sweat using surface-enhanced Raman scattering(SERS)technology.The researchers addressed issues such as poor stability caused by signal fluctuations by developing a novel sensor called the petal-like gap-en-hanced Raman tag(PGERT).The sensor utilizes 4-mercaptobenzonitrile(4-MBN)molecules internal-ly for an internal standard Raman signal and externally modifies the surface with 4-mercaptobenzoic acid(4-MBA)pH-sensing molecules to enhance the probe signal.Through in situ analysis,the study established standard analysis curves under pH conditions ranging from 5.0 to 8.0,achieving a high correlation coefficient(r2)of up to 0.99.The sensor demonstrated high stability and sensitivity.Error rates between the sensor′s detection results for sweat at different pH levels(pH 5.0 to 7.0)and stan-dard pH meter measurements were(2.17±5.0)%,(4.62±1.1)%,(5.80±0.3)%and(3.68±1.0)%,respectively.These results indicate that the sensor,based on the internal standard PGERT,can be applied for monitoring pH levels in human sweat,offering advantages such as high sensitivity,stability,and precise analysis.

关 键 词：花瓣状间隙增强拉曼标签(PGERT) 内标 PH检测 汗液 SERS 

分 类 号：O657.3[理学—分析化学] TP212.3[理学—化学]