基于表型性状构建禾初级核心种质  

Construction of Primary Core Collection of Rice‘HE’Based on Phenotypic Traits

作　　者：毛名义 杨文荟 管艳伟 潘宗东 周丽洁 余显权[1,2] MAO Mingyi;YANG Wenhui;GUAN Yanwei;PAN Zongdong;ZHOU Lijie;YU Xianquan(College of Agronomy,Guizhou University,Guiyang 550025,China;Institute of Rice Industry Technology Research,Guizhou University,Guiyang 550025,China;Qiandongnan Academy of Agricultural Sciences,Kaili,Guizhou 556000,China)

机构地区：[1]贵州大学农学院,贵阳550025 [2]贵州大学水稻产业技术研究院,贵阳550025 [3]黔东南州农业科学研究院,贵州凯里556000

出　　处：《中国稻米》2024年第1期18-25,共8页China Rice

基　　金：国家自然科学基金(32260444);贵州省粮油作物分子育种重点实验室项目(黔科合中引地[2023]008);贵州省科技计划项目(黔科合支撑[2022]重点026);贵州省科技计划项目(黔科合成果[2023]一般033);贵州省高等学校功能农业重点实验室项目(黔教技[2023]007号)。

摘　　要：“禾”是适应贵州黔东南山区峡谷森林中遮荫、泥沼、寒冷、积水等特殊自然生境生长的地方水稻品种。以402份禾种质资源为材料,依据禾颖尖颜色差异将原始种质划分为5组,基于26个表型性状,采用逐步聚类法以6种总体取样规模、4种组内取样比例和2种组内取样方法筛选最佳取样策略,构建禾初级核心种质,并对其进行综合评价。结果表明,10%为最适总体取样规模,组内取样比例法以对数比例法最优,聚类取样法是组内最佳取样方法。在最佳取样策略下,共获得47份禾初级核心种质,占比11.69%,表型保留比例为95.77%。核心种质评价结果表明,构建的核心种质作为原始种质的浓缩能够代表禾原始种质的遗传多样性。‘HE’is a rice landraces that adapts to the special natural habitat of shading,slough,cold and waterlogged in deep-valley and forests in the Qiandongnan mountainous areas in Guizhou.In this study,402 HE resources,divided into 5 groups based on the color differences of the glume tip,were used as materials.Based on the 26 phenotypic traits,the progressive clustering method was applied to screen out the optional sampling strategy by using 6 overall sampling scales,4 intra-group sampling ratios and 2 intra-group sampling methods,and to construct the primary core collection and evaluate it comprehensively.The results showed that 10%was the optimal overall sampling scale,the optimal intra-group sampling ratio method was the logarithmic ratio method,and the best intra-group sampling method was the cluster sampling method.Under the optimal sampling strategy,47 primary core collections of HE was obtained,accounting for 11.69%of the whole germplasm,and the retention rate of phenotype was 95.77%.The core collection constructed in this study could be a good representation of the genetic diversity of the original germplasm as a concentration of it.

关 键 词：水稻 禾 表型性状 初级核心种质 取样策略 

分 类 号：S511.02[农业科学—作物学]