基于rGO-AuNPS修饰丝网印刷电极的电化学生物传感器快速检测甲基对硫磷  

Rapid detection of methyl parathion by electrochemical biosensor based on rGO-AuNPS modified screen printing electrode

作　　者：耿俊豪 李雪芝[1] 周建平[1] 陈昌华 GENG Junhao;LI Xuezhi;ZHOU Jianping;CHEN Changhua(College of Intelligent Manufacturing Modern Industry,Xinjiang University,Urumqi,Xinjiang 830000,China)

机构地区：[1]新疆大学智能制造现代产业学院,新疆乌鲁木齐830000

出　　处：《食品与机械》2024年第1期47-54,共8页Food and Machinery

基　　金：新疆维吾尔自治区自然科学基金(编号:2021D01C102);新疆维吾尔自治区博士研究生科研创新项目(编号:XJ2021G048)。

摘　　要：目的:实现对甲基对硫磷的快速检测。方法:基于金纳米颗粒和还原氧化石墨烯制备了用于对甲基对硫磷进行定量检测的乙酰胆碱酯酶传感器。采用层层组装方法,将还原氧化石墨烯、金纳米颗粒、乙酰胆碱酯酶依次修饰在丝网印刷电极表面。对传感器的催化活性、阻抗特性、传感器的抑制率与甲基对硫磷浓度的关系、实际样品检测进行了评估。结果:制备的乙酰胆碱酯酶生物传感器对乙酰硫代胆碱氯化物表现出优异的亲和力,米氏常数为2.76 mmol/L。在最佳条件下,可以有效检测甲基对硫磷,线性范围为5~500 ng/mL,检出限为0.692 ng/mL。结论:该方法操作简单、成本低、稳定性好,适用于有机磷类农药的快速检测。Objective:In order to achieve rapid detection of methyl parathion.Methods:An acetylcholinesterase sensor was prepared based on gold nanoparticles and reduced graphene oxide for quantitative detection of Methyl parathion.Reduced graphene oxide,gold nanoparticles and acetylcholinesterase were successively modified on the surface of the screen printing electrode by layer-by-layer assembly method.The catalytic activity and impedance characteristics of the sensor,the relationship between the sensor s inhibition rate and MP concentration,and the actual sample detection were evaluated.Results:The prepared acetylcholinesterase biosensors showed excellent affinity for acetylthiocholine chloride with the Michaelis-Menten constant of 2.76 mmol/L.Under the optimal conditions,Methylparathion could be effectively detected with a linear range of 5 ng/mL to 500 ng/mL and a detection limit of 0.692 ng/mL.Conclusion:The method is simple,practical and stable.It provides a reliable method for rapid detection of organophosphorus pesticides.

关 键 词：生物传感器 丝网印刷电极 甲基对硫磷 还原氧化石墨烯 纳米金 

分 类 号：O657.1[理学—分析化学]