净化柱结合超高效液相色谱—串联质谱检测动物源性食品中16种喹诺酮药物  

Establishment and application of clean-up combined with ultra-high performance liquid chromatography tandem mass spectrometry for the detection of 16 quinolones in animal-derived food

作　　者：余婷婷 梁松 黄文慧 何洁 严义勇 YU Tingting;LIANG Song;HUANG Wenhui;HE Jie;YAN Yiyong(Shenzhen Bioeasy Biotechnology Co.,Ltd.,Shenzhen,Guangdong 518101,China;Institute of Critical Materials for Integrated Circuits,Shenzhen Polytechnic,Shenzhen,Guangdong 518055,China)

机构地区：[1]深圳市易瑞生物技术股份有限公司,广东深圳518101 [2]深圳职业技术学院集成电路关键材料研究院,广东深圳518055

出　　处：《食品与机械》2024年第1期55-62,67,共9页Food and Machinery

基　　金：广东省粤港湾联合创新领域项目(编号:2021A0505080003);资助深圳市技术攻关重点项目(编号:JSGG20191115141601721)。

摘　　要：目的:加强动物源性食品安全检测市场监管。方法:动物源性食品样品使用80%乙腈(含0.2%甲酸)提取,净化柱(Speedy Prep-Quino 1)净化后,利用超高效液相色谱—串联质谱检测16种喹诺酮类药物残留。结果:16种喹诺酮类药物的线性范围为1.6~40.0μg/kg,相关系数r≥0.9961,检出限为0.14~0.80μg/kg,定量限为0.47~2.68μg/kg。在净化柱前处理后7个基质加标回收率为62%~112%,相对标准偏差为0.9%~18.7%。结论:该方法具有检测速度快、灵敏度高的特点,适用于羊肉、鸭肉、牛肉、鱼肉、鸡蛋、猪腰、鸭皮等动物源性食品。Objective:A method for simultaneous detection of 16 quinolones in food of animal origin was developed byclean-up column pretreatment combined with ultra high performance liquid chromatography-tandem mass spectrometry.Methods:Food samples of animal origin were extracted using 80%acetonitrile(containing 0.2%formic acid).After purification on a Speedy Prep-Quino 1 column,16 quinolone residues were detected by ultra-high performance liquid chromatography tandem mass spectrometry.Results:The results showed that the linear range of 16 quinolones was 1.6~40.0μg/kg,the correlation coefficient r≥0.9961,the limits of detection were 0.14~0.80μg/kg,and the limits of quantification were 0.47~2.68μg/kg.The recoveries of seven matrix after pretreatment were 62%~112%,and the relative standard deviations were 0.9%~18.7%.Conclusion:The method has the characteristics of fast detection speed and high sensitivity,and can be applied to animal derived food such as mutton,duck,beef,fish,eggs,pig kidney,duck skin.

关 键 词：动物源性食品 喹诺酮 净化柱前处理 超高效液相色谱—串联质谱 

分 类 号：O657.63[理学—分析化学] TS207.3[理学—化学]