高效液相色谱法测定抗氧化剂BHT及其氧化产物BHTOOH的含量  

Determination of Antioxidant BHT and its Oxidation Product BHTOOH by High Performance Liquid Chromatography

作　　者：吴慧珊 唐菁 宁新霞 仇雪逸 戴素仪 赖芳[1] 刘雄民[1] 马丽[1] WU Huishan;TANG Jing;NING Xinxia;QIU Xueyi;DAI Suyi;LAI Fang;LIU Xiongmin;MA Li(School of Chemistry and Chemical Engineering of Guangxi University,Nanning 530004,China)

机构地区：[1]广西大学化学化工学院,广西南宁530000

出　　处：《化工技术与开发》2024年第1期67-71,共5页Technology & Development of Chemical Industry

基　　金：自治区大学生创新创业计划项目(S202210593297)。

摘　　要：对抗氧化剂BHT的使用安全性进行定量评估时,需要建立一种检测BHT及其过氧化产物BHTOOH含量的分析方法。本文采用Hypersil^(TM)ODS-2 C18色谱柱进行检测,色谱条件为:流动相为乙腈∶水=65∶35(体积比),流速1mL·min^(-1),检测波长221nm。结果表明,BHT及BHTOOH分别在0.2~1.2mg·mL^(-1)和0.02~0.12mg·mL^(-1)范围内线性关系良好(0.9991≤R^(2)≤0.9996),检出限(LOD,S/N=3)分别为0.0033mg·mL^(-1)和0.0029mg·mL^(-1),精密度高且均在6h内稳定存在,加标回收率分别为98.16%~100.3%和99.05%~102.5%,RSD分别为1.28%和1.74%。本方法可快速准确地对BHT及其过氧化物BHTOOH进行定量分析。For quantitative assessment of the safety of antioxidant BHT,an analytical method for detecting the content of BHT and its peroxidation product BHTOOH was established.The separation was performed on a Hypersil^(TM)ODS-2 C18 column.The mobile phase was acetonitrile and water in the ratio of 65:35(V:V),and the mobile phase flow rate was 1 mL/min.The detection wavelength was 221nm.The results showed that the linearity of BHT and BHTOOH were good in the ranges of 0.2~1.2 mg/mL and 0.02~0.12 mg/mL(0.9991≤R^(2)≤0.9996),and the limits of detection(LOD,S/N=3)were 0.0033mg/mL and 0.0029mg/mL,respectively.The calibration curves were stable within 6h.The spiked recoveries were 98.16%~100.3% and 99.05%~102.5% with RSDs of 1.28% and 1.74%,respectively.The method could be used to quantify BHT and its peroxide BHTOOH rapidly and accurately.

关 键 词：BHT 安全性评估 高效液相色谱法 定量分析 

分 类 号：O657.72[理学—分析化学]