定悸方干预心力衰竭模型大鼠心室重构机制研究  

Study on Effect of Dingji Formula(定悸方)on Ventricular Remodeling in Rats with Heart Failure and Its Mechanism

作　　者：诸燕英 张振贤[1] 王浩 陈若宏 黄瑶[1] ZHU Yanying;ZHANG Zhenxian;WANG Hao;CHEN Ruohong;HUANG Yao(Department of Traditional Chinese Internal Medicine,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China)

机构地区：[1]上海中医药大学附属岳阳中西医结合医院中医内科,上海200437

出　　处：《辽宁中医杂志》2023年第11期224-229,I0003,共7页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81603594);上海卫计委资助项目(201740151);上海市中医药事业发展三年行动计划项目(2018—2020年);治未病科服务能力建设项目[ZY(2018-2020)-ZWB-1001]。

摘　　要：目的探讨定悸方干预心力衰竭模型大鼠心室重构作用机制,同时观察定悸方与定悸方益气养阴组分组之间的疗效差异。方法采用异丙肾上腺素诱导建立心力衰竭大鼠,分别给予蒸馏水(模型组)、定悸方(定悸方组)、定悸方益气养阴组分组(益气养阴组分组)、倍他乐克(倍他乐克组)灌胃给药治疗6周后,心超观察大鼠心脏功能及结构改变,HE染色法观察心脏组织形态学变化,免疫组化法检测心肌组织中炎症因子肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)的表达情况,蛋白质印迹法检测大鼠心脏样本中TLR4、NF-κB p65蛋白表达情况。结果(1)与模型组比较,益气养阴组分组与定悸方组大鼠左室间隔及左室后壁厚度、左室内径均有明显下降(P<0.01),射血分数有明显提升(P<0.01)。与模型组比较,各治疗组大鼠心室重构情况均出现明显改善。(2)与模型组比较,益气养阴组分组、定悸方组大鼠心肌组织中炎症因子TNF-α、IL-1β以及IL-6均有下降,其中IL-1β的下降程度更为显著(P<0.01),且定悸方在下调IL-1β表达方面优于益气养阴组分组(P<0.01)。(3)与模型组比较,益气养阴组分组、定悸方组大鼠心肌组织中TLR4、NF-κB p65表达均下降(P<0.01),定悸方下调TLR4、NF-κB p65表达的效果优于益气养阴组分组,但差异无统计学意义(P>0.05)。结论定悸方能通过降低IL-1β、IL-6、TNF-α、TLR4、NF-κB p65蛋白的表达,减少炎症因子的释放,改善心室重构的作用,且相对单用定悸方益气养阴组分,定悸方疗效更好。Objective To study the effect of Dingji Formula(定悸方)on ventricular remodeling in rats with heart failure and its mechanism and observe the difference in curative effect between Dingji Formula and the components of benefiting Qi and nour-ishing Yin in Dingji Formula.Methods Rats with heart failure were induced by isoproterenol and treated with distilled water(mod-el group),Dingzhe Formula(Dingzhe Formula group),components of benefiting Qi and nourishing Yin in Dingji Formula(benefi-ting Qi and nourishing Yin group)and Betaloc(Betaloc group)by gavage for 6 weeks.Cardiac function and structural changes were observed by echocardiography.The morphological changes in cardiac tissue were observed by HE staining,and inflammatory factors[tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and interleukin-6(IL-6)]in myocardial tissue were detected by immunohistochemistry and the protein expressions of TLR4 and NF-κB p65 in the heart samples were detected by u-sing Western blotting.Results 1.Compared with those of the model group,left ventricular septum and left ventricular posterior wall thickness and left ventricular diameter of rats in the benefiting Qi and nourishing Yin group and Dingji Formula group were signifi-cantly decreased(P<0.01),and the ejection fraction was increased(P<0.01).In comparison with the model group,ventricular remodeling in rats in all treatment groups showed significant improvement.2.In comparison with those of the model group,the lev-els of TNF-α,IL-1βand IL-6 decreased in both the Dingji Fang group and the benefiting Qi and nourishing Yin group,and the decrease of IL-1βhad statistical difference(P<0.01).Compared with the benefiting Qi and nourishing Yin group,Dingji Formula group was slightly better in down-regulating the expression of IL-1β(P<0.01).3.In comparison with those of the model group,the expressions of TLR4 and NF-κB p65 in the myocardium in the benefiting Qi and nourishing Yin group and Dingji Formula group were decreased(P<0.01).Dingji Formula's effect of down-regula

关 键 词：心室重构 炎症因子 TLR4/NF-κB信号通路 定悸方 

分 类 号：R256.21[医药卫生—中医内科学]