疏肝调神和胃针法对慢性偏头痛大鼠模型血浆5-HT、CGRP、VIP的影响  

Effects of Soothing Liver,Regulating Mind and Harmonizing Stomach Acupuncture on Plasma 5-HT,CGRP and VIP in Rats with Chronic Migraine

作　　者：黄丽霞 罗倩 张俊萍 张玉莹 韩晶 杨佃会[1,2,3] HUANG Lixia;LUO Qian;ZHANG Junping;ZHANG Yuying;HAN Jing;YANG Dianhui(Shandong University of Traditional Chinese Medicine,Jinan 250355,Shandong,China;Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250014,Shandong,China;SHAN Qiuhua National Traditional Chinese Medicine Experts Inheritance Studio,Jinan 250014,Shandong,China)

机构地区：[1]山东中医药大学针灸推拿学院,山东济南250355 [2]山东中医药大学附属医院,山东济南250014 [3]单秋华全国名老中医药专家传承工作室,山东济南250014

出　　处：《辽宁中医杂志》2023年第11期230-233,I0004,共5页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81503502);2020年齐鲁中医学术流派传承项目(鲁卫函[2020]132号);山东省自然科学基金项目(ZR2020MH365)。

摘　　要：目的观察疏肝调神和胃针法对偏头痛大鼠体质量、热痛阈值及5-羟色胺(5-HT)、降钙素基因相关肽(CGRP)、血管活性肠肽(VIP)的影响,探讨疏肝调神和胃针法治疗慢性偏头痛可能的作用机制。方法将32只雄性大鼠随机分为正常对照组、模型组、普通针刺组、疏肝调神和胃组,每组8只。隔日(第1、3、5、7、9天)采用大鼠腹腔注射硝酸甘油制备慢性偏头痛模型。每日固定30 min,疏肝调神和胃组针刺“百会”及“内关”(双)、“足三里”(双)、“太冲”(双),普通针刺组针刺“风池”(双)、“太阳”(双)、“外关”(双),余组只固定不针刺,连续9 d。测定造模及治疗后大鼠体质量、热痛阈值的变化。采用ELISA法检测各组血浆中5-HT、CGRP、VIP的浓度。结果造模成功后,CM模型大鼠体质量增长缓慢、热痛阈值降低,与正常对照组比,差异具有统计学意义(P<0.05)。普通针刺组与疏肝调神和胃组体质量及热痛阈值增加,与模型组相比,差异有明显统计学意义(P<0.05)。普通针刺组与疏肝调神和胃组对比有差异,但无统计学意义,从趋势上看,疏肝调神和胃组优于普通针刺组。与正常对照组比较,模型组血浆5-HT含量明显降低(P<0.01),CGRP、VIP的含量明显增加(P<0.01)。与模型组比较,疏肝调神和胃组、普通针刺组大鼠血浆5-HT含量明显升高(P<0.01),CGRP、VIP的浓度明显降低(P<0.01)。结论疏肝调神和胃针法在抑制慢性偏头痛大鼠血浆5-HT、CGRP、VIP因子的痛觉传导更具优势,能减缓偏头痛疼痛慢性化进程,是针灸治疗慢性偏头痛的作用机制。Objective To observe the effects of soothing liver,regulating mind and harmonizing stomach acupuncture on body weight,thermal pain threshold,serotonin(5-HT),calcitonin gene-related peptide(CGRP)and vasoactive intestinal peptide(VIP)in migraine rats and explore the possible mechanism of the treatment of chronic migraine by soothing liver,regulating mind and harmonizing stomach acupuncture.Methods Thirty-two male rats were randomly divided into normal control group,model group,common acupuncture group,soothing liver,regulating mind and harmonizing stomach group,with 8 rats in each group.The chronic migraine model was established by intraperitoneal injection of nitroglycerin in rats on every other day(day 1,3,5,7 and 9).The rats were daily fixed for 30 minutes.The soothing liver,regulating mind and harmonizing stomach group used acupuncture at“Baihui(DU20)”and“Neiguan(PC6)”(double),“Zusanli(ST36)”(double)and“Taichong(LR3)”(double).The com-mon acupuncture group used acupuncture at“Fengchi(GB20)”(double),“Taiyang(EX-HN5)”(double)and“Waiguan(SJ5)”(double).The remaining groups were only fixed without acupuncture for 9 days.The changes of body weight and thermal pain threshold of rats after modeling and treatment were measured.The concentrations of 5-HT,CGRP and VIP in the plasma of each group were detected by ELISA.Results After successful modeling,the body weight of chronic migraine model rats increased slowly and the thermal pain threshold decreased,and the difference was significant compared with those of the normal control group(P<0.05).Compared with those of the model group,the body weight and thermal pain threshold increased in the common acupuncture group and the soothing liver,regulating mind and harmonizing stomach group(P<0.05).There were differences be-tween the common acupuncture group and the soothing liver,regulating mind and harmonizing stomach group,but there was no sig-nificant difference.From the trend,the soothing liver,regulating mind and harmonizing stomach group was bett

关 键 词：针刺 慢性偏头痛 5-羟色胺 降钙素基因相关肽 血管活性肠肽 

分 类 号：R246.6[医药卫生—针灸推拿学]