苯醚甲环唑对感靶斑病烟叶叶际微生物群落多样性的影响  

Effect of Difenoconazole on the Diversity of Phyllosphere Microbial Communities of Leaf Infected with Tobacco Taget Spot

作　　者：吴小军 汪汉成 林木森 彭丽娟 蔡刘体 陈兴江 林丽 郭涛 WU Xiaojun;WANG Hancheng;LIN Musen;PENG Lijuan;CAI Liuti;CHEN Xingjiang;LIN Li;GUO Tao(College of Tobacco,Guizhou University,Guiyang 550025,China;Guizhou Academy of Tobacco Science,Guiyang 550081,China;Daozhen Municipal Company of Guizhou Tobacco Company,Daozhen Guizhou 563500,China;College of Life Science,Yangtze University,Jingzhou Hubei 434025,China)

机构地区：[1]贵州大学烟草学院,贵阳550025 [2]贵州省烟草科学研究院,贵阳550081 [3]贵州烟草公司道真县公司,贵州道真563500 [4]长江大学生命科学学院,湖北荆州434025

出　　处：《植物医学》2023年第6期1-12,共12页Plant Health and Medicine

基　　金：中国烟草总公司科技项目[110202101048(LS-08),110202001035(LS-04)];国家自然科学基金(32160522,31960550);贵州省“百层次”创新型人才项目(黔科合平台人才-GCC〔2022〕028-1);贵州省科技基金项目(黔科合基础-ZK〔2021〕重点036).

摘　　要：为了解感靶斑病烟叶叶际微生物群落在苯醚甲环唑处理后的变化规律,本文采用高通量测序技术对苯醚甲环唑处理后不同时期感靶斑病烟叶与健康烟叶叶际微生物的群落结构和多样性进行分析.结果表明,相较于处理前,感病与健康烟叶叶际优势真菌属和细菌属的种类均增加,优势真菌属均分布于担子菌门和子囊菌门,优势细菌属均分布于变形菌门.在苯醚甲环唑(90 g/hm 2)处理后1~18 d,感病与健康烟叶叶际优势真菌属和细菌属相对丰度在不同时间点存在差异,但整体变化趋势一致.在真菌方面,亡隔菌属(Thanatephorus)相对丰度呈降低—增加—降低趋势;Plectosphaerella、Sampaiozyma、Symmetrospora、Pleurotus和链隔孢属(Alternaria)相对丰度呈增加—降低趋势;尾孢属(Cercospora)相对丰度呈降低趋势.在细菌方面,假单胞菌属(Pseudomonas)、鞘氨醇单胞菌属(Sphingomonas)和短小杆菌属(Curtobacterium)相对丰度呈增加—降低—增加—降低趋势;甲基杆菌属(Methylobacterium)和泛菌属(Pantoea)相对丰度均呈增加—降低趋势.感病与健康烟叶叶际真菌和细菌群落的多样性和丰富度同步变化,均整体呈增加—降低趋势,感病与健康烟叶叶际真菌群落多样性和丰富度的差异逐渐缩小.综上,苯醚甲环唑处理后感病与健康烟叶叶际真菌和细菌群落结构和多样性整体变化趋势相似,且随时间延长感病与健康烟叶叶际真菌和细菌群落差异缩小.In order to investigate the changing patterns of the phyllosphere microbial community of tobacco infected with tobacco target spot after difenoconazole treatment,this paper analyzed the community structure and diversity of phyllosphere microbial communities in diseased and healthy tobacco leaves at different periods after difenoconazole treatment by using high-throughput sequencing technology.The results showed that the number of dominant genera of fungi and bacterium increased in the diseased and healthy tobacco leaves compared to those before treatment.The dominant genera of fungi were distributed in Basidiomycota and Ascomycota,and the dominant genera of bacterium were distributed in Proteobacteria.The relative abundance of dominant genera of fungi and bacterium in the diseased and healthy tobacco leaves differed at different time points from 1 to 18 days after difenoconazole(90 g/hm 2)treatment,but the overall trend was consistent.In the fungal community,the relative abundance was in a decreasing-increasing-decreasing trend for Thanatephorus,an increasing-decreasing trend for Plectosphaerella,Sampaiozyma,Symmetrospora,Pleurotus and Alternaria,and a decreasing trend for Cercospora.In the bacterial community,the relative abundance was in an increasing-decreasing-increasing-decreasing trend for Pseudomonas,Sphingomonas and Curtobacterium,and an increasing-decreasing trend for Pantoea and Methylobacterium.The diversity and richness of the phyllosphere fungal and bacterial communities of diseased and healthy tobacco leaves changed synchronously,with an overall increasing-decreasing trend,and the differences in the diversity and richness of the phyllosphere fungal communities of diseased and healthy tobacco gradually decreased.In summary,the overall changes in the structure and diversity of the phyllosphere fungal and bacterial communities in diseased and healthy tobacco were similar after difenoconazole treatment,and the differences in the phyllosphere fungal and bacterial communities between diseased and healthy

关 键 词：苯醚甲环唑 烟草靶斑病 叶际 微生物群落 多样性 高通量测序 

分 类 号：S154.3[农业科学—土壤学] S572[农业科学—农业基础科学]