无锡地区HBsAg-/HBV DNA+献血人群HBcrAg检出特点分析  

Characteristics of HBcrAg in HBsAg-/HBV DNA+blood donors inWuxi area

作　　者：王嫣 金建怀 许友山 郝庆钦 夏卫 WANG Yan;JIN Jianhuai;XU Youshan;HAO Qingqin;XIA Wei(Wuxi Central Blood Station,Wuxi 214000,China)

机构地区：[1]无锡市中心血站,江苏无锡214000

出　　处：《中国输血杂志》2024年第1期32-36,共5页Chinese Journal of Blood Transfusion

基　　金：江苏省无锡市卫生健康委科研青年项目(Q201928)。

摘　　要：目的分析新型血清标志物乙型肝炎核心相关抗原(HBcrAg)在无锡地区HBsAg-/HBV DNA+献血人群中的检出特点。方法通过电话追踪随访了37名既往HBsAg-/HBV DNA+献血者并获得其血清,采用电化学发光法和实时荧光定量PCR核酸筛检出22例HBsAg-/HBV DNA+献血者血清作为OBI组进行HBcrAg酶联免疫吸附法检测。挑选出20名经2遍酶免和1遍核酸筛检的健康献血者的血清作为健康对照组,20例经无锡第五人民医院临床诊断为慢性乙型肝炎患者血清作为实验的CHB组,分别进行HBcrAg酶联免疫吸附法检测;并对OBI组进行HBcrAg与HBeAb、HBcAb、ALT、HBV DNA的相关性分析。结果37份献血者标本经化学发光法检测HBsAg和核酸筛查,检出22份HBsAg-/HBV DNA+标本即OBI组,检出率59.46%。OBI组与健康对照组、CHB组血清的HBcrAg表达含量分别是(0.92±0.13)ng/mL、(0.47±0.09)ng/mL、(1.14±0.23)ng/mL(P<0.05),OBI组与CHB组的HBcrAg表达均高于健康对照组(P<0.05)。OBI组的HBcrAg与HBeAb、HBcAb、ALT、HBV DNA指标均无相关性(P>0.05)。结论OBI组与CHB组的HBcrAg表达均高于健康对照组,其血清HBcrAg在一定程度上与HBeAb、HBcAb、ALT、HBV DNA无相关性,HBcrAg在筛查HBsAg-/HBV DNA+献血者中具有较好的应用前景。Objective To analyze the detection characteristics of a novel serum marker,hepatitis B core-associated antigen(HBcrAg),in the HBsAg-/HBV DNA+blood donors in Wuxi.Methods A total of 37 previous HBsAg-/HBV DNA+blood donors were followed up by telephone and their serum was obtained,and the serum of 22 HBsAg-/HBV DNA+blood donors was detected by electrochemiluminescence and real-time PCR nucleic acid screening as the OBI group for HBcrAg enzyme-linked immunosorbent assay(ELISA).The serum of 20 healthy blood donors who underwent dual ELISA and one nucleic acid testing(NAT)was selected as the healthy control group,and the serum of 20 patients with chronic hepatitis B who were clinically diagnosed by Wuxi Fifth People's Hospital was selected as the experimental CHB group,and HBcrAg ELISA was detected respectively.The correlation analysis between HBcrAg and HBeAb,HBcAb,ALT and HBV DNA in the OBI group was performed.Results Thirty-seven blood samples were detected by chemiluminescence for HBsAg and NAT,and 22 HBsAg-/HBV DNA+samples were detected in the OBI group,with a detection rate of 59.46%.The serum HBcrAg expression content(ng/mL)between the OBI group,the healthy control group and the CHB group were(0.92±0.13),(0.47±0.09)and(1.14±0.23),respectively,and the differences were statistically significant(P<0.05),the expression of HBcrAg in the OBI group and CHB group was higher than that in the healthy control group(P<0.05).There was no correlation between HBcrAg and HBeAb,HBcAb,ALT and HBV DNA indexes in the OBI group(P>0.05).Conclusion The expression of HBcrAg in the OBI group and CHB group was higher than that in the healthy control group,and the serum HBcrAg was not correlated with HBeAb,HBcAb,ALT and HBV DNA to a certain extent.HBcrAg has a good application prospect in screening HBsAg-/HBV DNA+blood donors.

关 键 词：乙型肝炎核心相关抗原 HBsAg-/HBV DNA+ 献血者 共价闭合环状DNA 

分 类 号：R457.1[医药卫生—治疗学] R446[医药卫生—临床医学] R373.2